1887

Abstract

The locations of two mutations that prevent adhesion of Pf0-1 to sand columns and seeds (, hesio) were identified. Both lie in a single gene showing homology to the NtrC/NifA family of transcription activators. The predicted 55 kDa protein encoded by is most closely related to activators involved in expression of flagellar proteins, consistent with the lack of flagella in strains. Constitutive expression restored motility and adhesion to an strain, demonstrating that the observed phenotypes are due to lack of AdnA and not a consequence of other mutations or polar effects of mutations in on other genes.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-147-2-355
2001-02-01
2019-10-21
Loading full text...

Full text loading...

/deliver/fulltext/micro/147/2/1470355a.html?itemId=/content/journal/micro/10.1099/00221287-147-2-355&mimeType=html&fmt=ahah

References

  1. Altschul, S. F., Gish, W., Miller, W., Myers, E. W. & Lipman, D. J. ( 1990; ). Basic local alignment search tool. J Mol Biol 215, 403-410.[CrossRef]
    [Google Scholar]
  2. Arora, S. K., Ritchings, B. W., Almira, E. C., Lory, S. & Ramphal, R. ( 1997; ). A transcriptional activator, FleQ, regulates mucin adhesion and flagellar gene expression in Pseudomonas aeruginosa in a cascade manner. J Bacteriol 179, 5574-5581.
    [Google Scholar]
  3. Arora, S. K., Ritchings, B. W., Almira, E. C., Lory, S. & Ramphal, R. ( 1998; ). The Pseudomonas aeruginosa flagellar cap protein, FliD, is responsible for mucin adhesion. Infect Immun 66, 1000-1007.
    [Google Scholar]
  4. Beji, A., Izard, D., Gavini, F., Leclerc, H., Leseine-Delstanche, M. & Krembel, J. ( 1987; ). A rapid chemical procedure for isolation and purification of chromosomal DNA from gram-negative bacilli. Anal Biochem 162, 18-23.[CrossRef]
    [Google Scholar]
  5. Blatny, J. M., Brautaset, T., Winther-Larsen, H. C., Karunakaran, P. & Valla, S. ( 1997; ). Improved broad-host-range RK2 vectors useful for high and low regulated gene expression levels in gram-negative bacteria. Plasmid 38, 35-51.[CrossRef]
    [Google Scholar]
  6. Compeau, G., Al-Achi, B. J., Platsouka, E. & Levy, S. B. ( 1988; ). Survival of rifampin-resistant mutants of Pseudomonas fluorescens and Pseudomonas putida in soil systems. Appl Environ Microbiol 54, 2432-2438.
    [Google Scholar]
  7. Costerton, J. W., Lewandowski, Z., Caldwell, D. E., Korber, D. R. & Lappin-Scott, H. M. ( 1995; ). Microbial biofilms. Annu Rev Microbiol 49, 711-745.[CrossRef]
    [Google Scholar]
  8. Costerton, J. W., Stewart, P. S. & Greenberg, E. P. ( 1999; ). Bacterial biofilms: a common cause of persistent infections. Science 284, 1318-1322.[CrossRef]
    [Google Scholar]
  9. DeFlaun, M. F., Tanzer, A. S., McAteer, A. L., Marshall, B. & Levy, S. B. ( 1990; ). Development of an adhesion assay and characterization of an adhesion-deficient mutant of Pseudomonas fluorescens. Appl Environ Microbiol 56, 112-119.
    [Google Scholar]
  10. DeFlaun, M. F., Marshall, B. M., Kulle, E.-P. & Levy, S. ( 1994; ). Tn5 insertion mutants of Pseudomonas fluorescens defective in adhesion to soil and seeds. Appl Environ Microbiol 60, 2637-2642.
    [Google Scholar]
  11. Ditta, G., Stanfield, S., Corbin, D. & Helinski, D. R. ( 1980; ). Broad host range DNA cloning system for gram-negative bacteria: construction of a gene bank of Rhizobium meliloti. Proc Natl Acad Sci USA 77, 7347-7351.[CrossRef]
    [Google Scholar]
  12. Hächler, H., Cohen, S. P. & Levy, S. B. ( 1991; ). marA, a regulated locus which controls expression of chromosomal multiple antibiotic resistance in Escherichia coli. J Bacteriol 173, 5532-5538.
    [Google Scholar]
  13. Helmann, J. D. ( 1991; ). Alternative sigma factors and the regulation of flagellar gene expression. Mol Microbiol 5, 2875-2882.[CrossRef]
    [Google Scholar]
  14. Keen, N. T., Tamaki, S., Kobayashi, D. & Trollinger, D. ( 1988; ). Improved broad-host-range plasmids for DNA cloning in gram-negative bacteria. Gene 70, 191-197.[CrossRef]
    [Google Scholar]
  15. Kim, Y.-K. & McCarter, L. L. ( 2000; ). Analysis of the polar flagella gene system of Vibrio parahaemolyticus. J Bacteriol 182, 3693-3704.[CrossRef]
    [Google Scholar]
  16. Klose, K. E. & Mekalanos, J. J. ( 1998a; ). Distinct roles of an alternative sigma factor during both free-swimming and colonizing phases of the Vibrio cholerae pathogenic cycle. Mol Microbiol 28, 501-520.[CrossRef]
    [Google Scholar]
  17. Klose, K. E. & Mekalanos, J. J. ( 1998b; ). Differential regulation of multiple flagellins in Vibrio cholerae. J Bacteriol 180, 303-316.
    [Google Scholar]
  18. Korber, D. R., Lawrence, J. R. & Caldwell, D. E. ( 1994; ). Effect of motility on surface colonization and reproductive success of Pseudomonas fluorescens in dual-dilution continuous culture and batch culture systems. Appl Environ Microbiol 60, 1421-1429.
    [Google Scholar]
  19. Kustu, S., North, A. K. & Weiss, D. S. ( 1991; ). Prokaryotic transcriptional enhancers and enhancer-binding proteins. Trends Biochem Sci 16, 397-402.[CrossRef]
    [Google Scholar]
  20. Lajoie, C. A., Zylstra, G. J., DeFlaun, M. F. & Strom, P. F. ( 1993; ). Development of field application vectors for bioremediation of soils contaminated with polychlorinated biphenyls. Appl Environ Microbiol 59, 1735-1741.
    [Google Scholar]
  21. de Lorenzo, V., Fernandez, S., Herrero, M., Jakubzik, U. & Timmis, K. N. ( 1993; ). Engineering of alkyl- and haloaromatic-responsive gene expression with mini-transposons containing regulated promoters of biodegradative pathways of Pseudomonas. Gene 130, 41-46.[CrossRef]
    [Google Scholar]
  22. Mermod, N., Ramos, J. L., Lehrbach, P. R. & Timmis, K. N. ( 1986; ). Vector for regulated expression of cloned genes in a wide range of gram-negative bacteria. J Bacteriol 167, 447-454.
    [Google Scholar]
  23. Morett, E. & Segovia, L. ( 1993; ). The sigma 54 bacterial enhancer-binding protein family: mechanism of action and phylogenetic relationship of their functional domains. J Bacteriol 175, 6067-6074.
    [Google Scholar]
  24. O’Toole, G. A. & Kolter, R. ( 1998a; ). Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol Microbiol 30, 295-304.[CrossRef]
    [Google Scholar]
  25. O’Toole, G. A. & Kolter, R. ( 1998b; ). Initiation of biofilm formation in Pseudomonas fluorescens WCS365 proceeds via multiple, convergent signalling pathways: a genetic analysis. Mol Microbiol 28, 449-461.[CrossRef]
    [Google Scholar]
  26. Piette, J. P. & Idziak, E. S. ( 1992; ). A model study of factors involved in adhesion of Pseudomonas fluorescens to meat. Appl Environ Microbiol 58, 2783-2791.
    [Google Scholar]
  27. Ramphal, R., Koo, L., Ishimoto, K. S., Totten, P. A., Lara, J. C. & Lory, S. ( 1991; ). Adhesion of Pseudomonas aeruginosa pilin-deficient mutants to mucin. Infect Immun 59, 1307-1311.
    [Google Scholar]
  28. Ritchings, B. W., Almira, E. C., Lory, S. & Ramphal, R. ( 1995; ). Cloning and phenotypic characterization of fleS and fleR, new response regulators of Pseudomonas aeruginosa which regulate motility and adhesion to mucin. Infect Immun 63, 4868-4876.
    [Google Scholar]
  29. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  30. Scharfman, A., Kroczynski, H., Carnoy, C., Van Brussel, E., Lamblin, G., Ramphal, R. & Roussel, P. ( 1996; ). Adhesion of Pseudomonas aeruginosa to respiratory mucins and expression of mucin-binding proteins are increased by limiting iron during growth. Infect Immun 64, 5417-5420.
    [Google Scholar]
  31. Simon, R., Priefer, U. & Pühler, A. ( 1983; ). A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram-negative bacteria. Bio/Technology 1, 784-791.[CrossRef]
    [Google Scholar]
  32. Simons, M., van der Bij, A. J., Brand, I., de Weger, L. A., Wijffelman, C. A. & Lugtenberg, B. J. ( 1996; ). Gnotobiotic system for studying rhizosphere colonization by plant growth-promoting Pseudomonas bacteria. Mol Plant-Microbe Interact 9, 600-607.[CrossRef]
    [Google Scholar]
  33. Stanier, R. Y., Palleroni, N. J. & Doudoroff, M. ( 1966; ). The aerobic pseudomonads: a taxonomic study. J Gen Microbiol 43, 159-271.[CrossRef]
    [Google Scholar]
  34. Stewart, B. J. & McCarter, L. L. ( 1996; ). Vibrio parahaemolyticus FlaJ, a homologue of FliS, is required for production of a flagellin. Mol Microbiol 20, 137-149.[CrossRef]
    [Google Scholar]
  35. Stock, J. B., Stock, A. M. & Mottonen, J. M. ( 1990; ). Signal transduction in bacteria. Nature 344, 395-400.[CrossRef]
    [Google Scholar]
  36. van Veen, J. A., van Overbeek, L. S. & van Elsas, J. D. ( 1997; ). Fate and activity of microorganisms introduced into soil. Microbiol Mol Biol Rev 61, 121-135.
    [Google Scholar]
  37. Williams, V. & Fletcher, M. ( 1996; ). Pseudomonas fluorescens adhesion and transport through porous media are affected by lipopolysaccharide composition. Appl Environ Microbiol 62, 100-104.
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-147-2-355
Loading
/content/journal/micro/10.1099/00221287-147-2-355
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error