1887

Abstract

The identification of environmentally regulated genes of by the use of iron-restricted conditions and by experimental infection of piglets is described. Eighteen unique iron-restriction-induced () genes and 22 unique -selected () genes of were found. None of the genes was exclusively expressed . Four genes were identical to four clones selected in piglets. Two genes were similar to genes for putative virulence factors. One of these genes was identical to the gene of virulent serotype 2 strains and the other showed homology to a gene encoding a fibronectin-binding protein of . Two additional genes showed homology to environmentally regulated genes previously identified by using an expression technology (IVET) selection system in other bacterial species. One of these showed similarity to the gene of , a key locus involved in the regulation of numerous virulence proteins. The promoter selection system described in this paper has been successfully used for the identification of many environmentally regulated genes potentially involved in the pathogenesis of infections in piglets.

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2001-02-01
2020-01-21
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