1887

Abstract

Several strains of the genus , which were isolated in Japan from pear trees with necrotic symptoms that resembled fire blight, and tentatively identified as , were reinvestigated for their relationship to the fire blight pathogen. These isolates produced ooze on slices of immature pears and were mucoid on MM2Cu agar plates, but did not synthesize levan and did not give the expected PCR signals with several primer pairs specific for . The isolates tested positive with PCR primers designed to detect the novel pear pathogen , which was isolated from Nashi pear trees in South Korea. The nucleotide sequence analysis of a DNA fragment preceding the gene cluster for exopolysaccharide synthesis revealed a closer relationship to than to . Plasmid profiles, protein patterns and genomic DNA analysed by PFGE after I and I digestion were different than . Experiments with strains of isolated from raspberry ( sp.), and also did not reveal a relationship between these bacteria and the Japanese strains. The latter are not identical to , but possess many similar features to this pathogen that causes Asian pear blight. It is concluded that pathogenic bacteria isolated in Japan from pear trees with symptoms resembling fire blight are possibly different from

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2001-11-01
2020-01-24
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