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Volume 147, Issue 11

Molecular comparison of pathogenic bacteria from pear trees in Japan and the fire blight pathogen Free

Abstract

Several strains of the genus , which were isolated in Japan from pear trees with necrotic symptoms that resembled fire blight, and tentatively identified as , were reinvestigated for their relationship to the fire blight pathogen. These isolates produced ooze on slices of immature pears and were mucoid on MM2Cu agar plates, but did not synthesize levan and did not give the expected PCR signals with several primer pairs specific for . The isolates tested positive with PCR primers designed to detect the novel pear pathogen , which was isolated from Nashi pear trees in South Korea. The nucleotide sequence analysis of a DNA fragment preceding the gene cluster for exopolysaccharide synthesis revealed a closer relationship to than to . Plasmid profiles, protein patterns and genomic DNA analysed by PFGE after I and I digestion were different than . Experiments with strains of isolated from raspberry ( sp.), and also did not reveal a relationship between these bacteria and the Japanese strains. The latter are not identical to , but possess many similar features to this pathogen that causes Asian pear blight. It is concluded that pathogenic bacteria isolated in Japan from pear trees with symptoms resembling fire blight are possibly different from

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  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Asian pear blight , EPS, exopolysaccharide , Erwinia pyrifoliae , ITS, intergenic transcribed spacer , nucleotide sequence alignment , PCR signals , PFGE and SSR, short sequence DNA repeat
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2001-11-01
2024-04-23
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References

  1. Beer, S. V., Kim, J.-H., Zumoff, C. H. & 7 other authors (1996). Characterization of bacteria that cause ‘bacterial shoot blight of pear’ in Japan. Acta Hortic 411, 179–181. [Google Scholar]
  2. Bereswill, S., Pahl, A., Bellemann, P., Zeller, W. & Geider, K. (1992). Sensitive and species-specific detection of Erwinia amylovora by polymerase chain reaction analysis. Appl Environ Microbiol 58, 3522-3526. [Google Scholar]
  3. Bereswill, S., Bugert, P., Bruchmüller, I. & Geider, K. (1995). Identification of Erwinia amylovora by PCR with chromosomal DNA. Appl Environ Microbiol 61, 2636-2642. [Google Scholar]
  4. Bereswill, S., Jock, S., Bellemann, P. & Geider, K. (1998). Identification of Erwinia amylovora by growth morphology on agar containing copper sulfate and by capsule staining with lectin. Plant Dis 82, 158-164.[CrossRef] [Google Scholar]
  5. Bugert, P. & Geider, K. (1995). Molecular analysis of the ams-operon required for exopolysaccharide synthesis of Erwinia amylovora. Mol Microbiol 15, 917-933.[CrossRef] [Google Scholar]
  6. Chung, Y. R., Brenner, D. J., Steigerwalt, A. G., Kim, B. S., Kim, H. T. & Cho, K. Y. (1993).Enterobacter pyrinus sp. nov., an organism associated with brown leaf spot disease of pear trees. Int J Syst Bacteriol 43, 157-161.[CrossRef] [Google Scholar]
  7. Falkenstein, H., Bellemann, P., Walter, S., Zeller, W. & Geider, K. (1988). Identification of Erwinia amylovora, the fireblight pathogen, by colony hybridization with DNA from plasmid pEA29. Appl Environ Microbiol 54, 2798-2802. [Google Scholar]
  8. Goto, M. (1992).Fundamentals of Bacterial Plant Pathology. San Diego: Academic Press.
  9. Hugh, R. & Leifson, E. (1953). The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various Gram-negative bacteria. J Bacteriol 66, 24. [Google Scholar]
  10. Jock, S., Rodoni, B., Gillings, M., Kim, W.-S., Copes, C., Merriman, P. & Geider, K. (2000). Screening of ornamental plants from the Botanic Gardens of Melbourne and Adelaide for the occurrence of Erwinia amylovora. Australas Plant Pathol 29, 120-128.[CrossRef] [Google Scholar]
  11. Kim, J.-H., Beer, S. V., Zumoff, C. H., Laby, R. J., Gustafson, H. L., Aldwinckle, H. S. & Tanii, A. (1996). Characterization of Erwinia amylovora strains from different hosts and geographical areas. Acta Hortic 411, 183-186. [Google Scholar]
  12. Kim, W.-S. & Geider, K. (1999). Analysis of variable short-sequence DNA repeats on the 29 kb plasmid of Erwinia amylovora strains. Eur J Plant Pathol 108, 703-713. [Google Scholar]
  13. Kim, W.-S., Gardan, L., Rhim, S.-L. & Geider, K. (1999).Erwinia pyrifoliae sp. nov., a novel pathogen affecting Asian pear trees (Pyrus pyrifolia Nakai). Int J Syst Bacteriol 49, 899-906.[CrossRef] [Google Scholar]
  14. Kim, W.-S., Jock, S., Paulin, J.-P., Rhim, S.-L. & Geider, K. (2001). Molecular detection and differentiation of Erwinia pyrifoliae and host range analysis of the Asian pear pathogen. Plant Dis 85 (in press).
  15. McGhee, G. C. & Jones, A. L. (2000). Complete nucleotide sequence of ubiquitous plasmid pEA29 from Erwinia amylovora strain Ea88: Gene organization and intraspecies variation. Appl Environ Microbiol 66, 4897-4907.[CrossRef] [Google Scholar]
  16. Nei, M. & Li, W.-H. (1979). Mathematical model for studying genetic variation in terms of restriction nucleases. Proc Natl Acad Sci USA 76, 5269-5273.[CrossRef] [Google Scholar]
  17. Rhim, S.-L., Völksch, B., Gardan, L., Paulin, J.-P., Langlotz, C., Kim, W.-S. & Geider, K. (1999).Erwinia pyrifoliae, an Erwinia species, different from Erwinia amylovora, causes a necrotic disease of Asian pear trees. Plant Pathol 48, 514-520.[CrossRef] [Google Scholar]
  18. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989).Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  19. Weisburg, W. G., Barns, S. M., Pelletier, D. A. & Lane, D. J. (1991). 16S ribosomal DNA amplification for phylogenetic study. J Bacteriol 173, 697-703. [Google Scholar]
  20. Zhang, Y. & Geider, K. (1997). Differentiation of Erwinia amylovora strains by pulsed-field gel electrophoresis. Appl Environ Microbiol 63, 4421-4426. [Google Scholar]
  21. Zhang, Y., Merighi, M., Bazzi, C. & Geider, K. (1998). Genomic analysis by pulsed-field gel electrophoresis of Erwinia amylovora strains from the Mediterranean region including Italy. J Plant Pathol 80, 225-232. [Google Scholar]
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Molecular comparison of pathogenic bacteria from pear trees in Japan and the fire blight pathogen Erwinia amylovora
Microbiology 147, 2951 (2001); https://doi.org/10.1099/00221287-147-11-2951
/content/journal/micro/10.1099/00221287-147-11-2951
/content/journal/micro/10.1099/00221287-147-11-2951
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