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Volume 147, Issue 10

Cytochrome oxidase contains an extra charged amino acid cluster in a new type of respiratory chain in the amino-acid-producing Gram-positive bacterium

The GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AB052748 and AB052749.

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Abstract

The membranes from cells contain a hydrophobic di-haem C protein as the cytochrome subunit of the new type of cytochrome complex (complex III in the respiratory chain) encoded by the operon [Sone, N., Nagata, K., Kojima, H., Tajima, J., Kodera, Y., Kanamaru, T., Noguchi, S. & Sakamoto, J. (2001). 1503, 279–290]. To characterize complex IV, cytochrome oxidase and its structural genes were isolated. The oxidase is of the cytochrome type, but mass spectrometry indicated that the haem is haem As, which contains a geranylgeranyl side-chain instead of a farnesyl group. The enzyme is a SoxM-type haem–copper oxidase composed of three subunits. Edman degradation and mass spectrometry suggested that the N-terminal signal sequence of subunit II is cleaved and that the new N-terminal cysteine residue is diacylglycerated, while neither subunit I nor subunit III is significantly modified. The genes for subunits II () and III () are located upstream of the operon, while that for subunit I () is located separately. The oxidase showed low enzyme activity with extrinsic substrates such as cytochromes from horse heart or yeast, and has the Cu-binding motif in its subunit II. A prominent structural feature is the insertion of an extra charged amino acid cluster between the β2 and β4 strands in the substrate-binding domain of subunit II. The β2–β4 loop of this oxidase is about 30 residues longer than that of major cytochrome oxidases from mitochondria and proteobacteria, and is rich in both acidic and basic residues. These findings suggest that the extra charged cluster may play a role in the interaction of the oxidase with the cytochrome subunit of the new type of complex.

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Keyword(s): cytochrome aa3 , DG, n-decyl-d-glucoside , dihaem cytochrome c , glutamate fermentation , high-G+C Gram-positive bacteria , MALDI, matrix-assisted laser desorption ionization , MEGA 9, n-nonanoyl N-metylglucamide; MEGA 10, n-decanoyl N-methylglucamide , PTH, phenylthiohydantoin and TMPD, N,N,N′,N′-tetramethyl-p-phenylenediamine
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2001-10-01
2025-01-23
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Cytochrome c oxidase contains an extra charged amino acid cluster in a new type of respiratory chain in the amino-acid-producing Gram-positive bacterium Corynebacterium glutamicumThe GenBank/EMBL/DDBJ accession numbers for the sequences reported in this paper are AB052748 and AB052749.
Microbiology 147, 2865 (2001); https://doi.org/10.1099/00221287-147-10-2865
/content/journal/micro/10.1099/00221287-147-10-2865
/content/journal/micro/10.1099/00221287-147-10-2865
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