RT Journal Article SR Electronic(1) A1 Wynn, James P A1 Hamid, Adil A A1 Li, Yonghua A1 Ratledge, ColinYR 2001 T1 Biochemical events leading to the diversion of carbon into storage lipids in the oleaginous fungi Mucor circinelloides and Mortierella alpina JF Microbiology, VO 147 IS 10 SP 2857 OP 2864 DO https://doi.org/10.1099/00221287-147-10-2857 PB Microbiology Society, SN 1465-2080, AB The biochemical events associated with the onset of lipid accumulation in Mucor circinelloides and Mortierella alpina, under conditions of nitrogen-limited growth, have been elucidated; they differ in key aspects from those described in oleaginous yeasts. The NAD+:isocitrate dehydrogenases of Mc. circinelloides and Mort. alpina were not absolutely dependent on AMP for activity. Furthermore, changes in the cellular adenine nucleotide pools and energy charge were different from those reported for oleaginous yeasts. In Mc. circinelloides ATP, ADP and AMP concentrations all decreased by 50% after nitrogen limitation, leading to a constant energy charge at the expense of the size of the total adenylate pool. Pyruvate carboxylase in Mc. circinelloides was cytosolic, having implications for the organization of lipid synthesis in filamentous fungi. As a result of the data obtained, a revised and more concerted mechanism for the initiation of storage lipid accumulation is put forward for filamentous fungi., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-10-2857