@article{mbs:/content/journal/micro/10.1099/00221287-147-10-2795, author = "Toesca, Isabelle and Perard, Catherine and Bouvier, Jean and Gutierrez, Claude and Conter, Annie", title = "The transcriptional activator NhaR is responsible for the osmotic induction of osmCp1, a promoter of the stress-inducible gene osmC in Escherichia coli", journal= "Microbiology", year = "2001", volume = "147", number = "10", pages = "2795-2803", doi = "https://doi.org/10.1099/00221287-147-10-2795", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-147-10-2795", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "osmoregulation", keywords = "NhaR", keywords = "transcriptional regulation", keywords = "bacterial promoters", abstract = "Two overlapping promoters, osmC p1 and osmC p2, direct the transcription of the osmC gene of Escherichia coli. The proximal promoter, osmC p2, is induced upon entry into stationary phase under the control of Eσs, the RNA polymerase that uses the σs (RpoS) sigma factor. Transcription from the distal promoter, osmC p1, is independent of σs. Previous analysis demonstrated that the osmolarity of the growth medium modulates expression of both promoters. The use of an E. coli genomic library showed that the cloned nhaR gene was able to stimulate transcription of an osmC–lac reporter fusion. NhaR is a positive regulator of the LysR family, previously identified as an activator of nhaA, a gene encoding a Na+/H+ antiporter involved in adaptation to Na+ and alkaline pH in E. coli and other enteric bacteria. NhaR was shown to activate only the expression of osmC p1 and to be necessary for the induction of this promoter by LiCl, NaCl and sucrose. Therefore, activation by NhaR is responsible for the osmotic induction of osmC p1. In contrast to its action on nhaA, NhaR activation of osmC p1 is independent of H-NS. Activation of osmC p1 by NhaR requires a site located just upstream of the atypical −35 region of the promoter.", }