1887

Abstract

Two overlapping promoters, and , direct the transcription of the gene of . The proximal promoter, , is induced upon entry into stationary phase under the control of Eσ, the RNA polymerase that uses the σ (RpoS) sigma factor. Transcription from the distal promoter, , is independent of σ. Previous analysis demonstrated that the osmolarity of the growth medium modulates expression of both promoters. The use of an genomic library showed that the cloned gene was able to stimulate transcription of an reporter fusion. NhaR is a positive regulator of the LysR family, previously identified as an activator of , a gene encoding a Na/H antiporter involved in adaptation to Na and alkaline pH in and other enteric bacteria. NhaR was shown to activate only the expression of and to be necessary for the induction of this promoter by LiCl, NaCl and sucrose. Therefore, activation by NhaR is responsible for the osmotic induction of . In contrast to its action on , NhaR activation of is independent of H-NS. Activation of by NhaR requires a site located just upstream of the atypical −35 region of the promoter.

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2001-10-01
2019-10-19
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