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Volume 147, Issue 1

Identification and molecular characterization of an efflux system involved in S12 multidrug resistance Free

Abstract

The authors previously described , an operon involved in proton-dependent solvent efflux in the solvent-tolerant S12. Recently, it was shown that organic solvents and not antibiotics induce this operon. In the present study, the authors characterize a new efflux pump, designated ArpABC, on the basis of two isolated chloramphenicol-sensitive transposon mutants. The operon is involved in the active efflux of multiple antibiotics, such as tetracycline, chloramphenicol, carbenicillin, streptomycin, erythromycin and novobiocin. The deduced amino acid sequences encoded by the three genes involved show a striking resemblance to proteins of the resistance/nodulation/cell division family, which are involved in both organic solvent and multiple drug efflux. These findings demonstrate that ArpABC is highly homologous to the MepABC and TtgABC efflux systems for organic solvents and multiple antibiotics. However, ArpABC does not contribute to organic solvent tolerance in S12 but is solely involved in multidrug resistance.

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Keyword(s): efflux , multidrug resistance , RND family, resistance/nodulation/cell division family and solvent tolerance
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2001-01-01
2024-04-19
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Identification and molecular characterization of an efflux system involved in Pseudomonas putida S12 multidrug resistance
Microbiology 147, 43 (2001); https://doi.org/10.1099/00221287-147-1-43
/content/journal/micro/10.1099/00221287-147-1-43
/content/journal/micro/10.1099/00221287-147-1-43
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