RT Journal Article SR Electronic(1) A1 Toujima, Saori A1 Kuwano, Koichi A1 Zhang, Ye A1 Fujimoto, Naoyuki A1 Hirama, Masahiro A1 Oishi, Tohru A1 Fukuda, Sumiko A1 Nagumo, Yoko A1 Imai, Hiroto A1 Kikuchi, Tsukasa A1 Arai, Sumio YR 2000 T1 Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells JF Microbiology, VO 146 IS 9 SP 2317 OP 2323 DO https://doi.org/10.1099/00221287-146-9-2317 PB Microbiology Society, SN 1465-2080, AB A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh–Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O-[2′-O-(α-D-glucopyranosyl)- 6′-O-acyl-α-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using 1H- and 13C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC 14 (17·8%), isoC14 (10·7%) and nC 16 (34·9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC14 acyl group was higher than those with nC14 and nC16 acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs. , UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-146-9-2317