%0 Journal Article %A Toujima, Saori %A Kuwano, Koichi %A Zhang, Ye %A Fujimoto, Naoyuki %A Hirama, Masahiro %A Oishi, Tohru %A Fukuda, Sumiko %A Nagumo, Yoko %A Imai, Hiroto %A Kikuchi, Tsukasa %A Arai, Sumio %T Binding of glycoglycerolipid derived from membranes of Acholeplasma laidlawii PG8 and synthetic analogues to lymphoid cells %D 2000 %J Microbiology, %V 146 %N 9 %P 2317-2323 %@ 1465-2080 %R https://doi.org/10.1099/00221287-146-9-2317 %K GAGDG, 3-O-[2′-O-(α-D-glucopyranosyl)- 6′-O-acyl-α-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol %K Acholeplasma laidlawii %K adherence %K glycoglycerolipids %K lymphoid cells %I Microbiology Society, %X A component that binds to human lymphoid cells was isolated from the membranes of Acholeplasma laidlawii PG8. The component was extracted using the Bligh–Dyer method and purified using a silica-gel column and TLC. The active component was identified as 3-O-[2′-O-(α-D-glucopyranosyl)- 6′-O-acyl-α-D-glucopyranosyl]-1,2-di-O- acyl-sn-glycerol (GAGDG) using 1H- and 13C-NMR and GC-MS. The compositions of the major saturated fatty acids were nC 14 (17·8%), isoC14 (10·7%) and nC 16 (34·9%) as determined by GC-MS. The amounts of unsaturated species were less than 10% of those of the corresponding saturated acids. GAGDGs which have three tetradecanoyl groups were synthesized. These synthetic GAGDGs, as well as GAGDGs derived from A. laidlawii membranes, had a high binding affinity for MOLT-4 and HUT-78 (human T cell lines), Raji (a B cell line), HL-60 (a monoblastoid cell line) and primary cultured human T cells. The binding affinities of GAGDGs with an isoC14 acyl group was higher than those with nC14 and nC16 acyl groups. The binding to lymphoid cells reveals a novel biological activity of GAGDGs. %U https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-146-9-2317