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Volume 146, Issue 9

Concerted evolution of duplicate genes in Free

Abstract

Campylobacters have two similar copies ( and ) of their flagellin gene. It has been hypothesized that the two copies can serve for antigenic phase variation. Analysis of polymorphisms within aligned multiple DNA sequences of the flagellin genes revealed high pairwise homoplasy indexes between / pairs that were not observed between any / pairings or / pairings. Thus it seems there are constraints on the sequence of that distinguish it from . Nevertheless, segments of the two genes that are highly variable between strains are conserved between the and copies of the genes within a strain. The patterns of synonymous and non-synonymous differences suggest that one segment of the flagellin sequence is under selective pressure at the amino acid sequence level. Another segment of the protein is maintained within a strain by conversion or recombination. Comparisons of strict consensus amino acid sequences did not reveal any motifs that are uniquely FlaA or FlaB, but there are differences between FlaA and FlaB in those amino acids available for post-translational modification. The observed pattern of concerted evolution of portions of a structural gene is an unusual finding in bacteria and should be searched for with other duplicated genes. Concerted evolution was unexpected for genes involved in phase variation since it minimizes the antigenic repertoire that can be expressed by a single clone in the face of the host immune response.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): Campylobacter , concerted evolution , DNA multiple-sequence analyses and flagellin
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2000-09-01
2025-01-14
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/content/journal/micro/10.1099/00221287-146-9-2283
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Concerted evolution of duplicate fla genes in Campylobacter
Microbiology 146, 2283 (2000); https://doi.org/10.1099/00221287-146-9-2283
/content/journal/micro/10.1099/00221287-146-9-2283
/content/journal/micro/10.1099/00221287-146-9-2283
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