1887

Abstract

In the biosynthesis of the exopolysaccharide galactoglucan (EPS II) is directed by the genes. The and gene products are homologous to components of type I secretion systems. ExpE1, the gene of which is located adjacent to and , was detected in cells and culture supernatants. ExpD1 and ExpD2 were required for the secretion of ExpE1, indicating that ExpE1 is secreted by a type I secretion system involving ExpD1 and ExpD2. ExpE1 contains 15 aspartate- and glycine-rich nonapeptide repeats that were suggested to bind Ca. The ability to bind Ca was demonstrated for a recombinant ExpE1 protein. Extracellular EPS II was not detected in cultures of non-polar , and deletion mutants implying that these three genes are required for biosynthesis or secretion of galactoglucan.

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2000-09-01
2020-03-28
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