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Volume 146, Issue 9

The synthesis of the bacteriocin sakacin A is a temperature-sensitive process regulated by a pheromone peptide through a three-component regulatory system Free

Abstract

Sakacin A is a bacteriocin produced by Lb706. The gene cluster () encompasses a regulatory unit composed of three consecutive genes, and . encode a histidine protein kinase and a response regulator, while encodes the putative precursor of a 23-amino-acid cationic peptide (termed Sap-Ph). The authors show that Sap-Ph serves as a pheromone regulating bacteriocin production. Lb706 produced bacteriocin when the growth temperature was kept at 25 or 30 °C, but production was reduced or absent at higher temperatures (335–35 °C). Production was restored by lowering the growth temperature to 30 °C, but at temperatures of 33–34 °C also by adding exogenous Sap-Ph to the growth medium. A knock-out mutation in abolished sakacin A production. Exogenously added Sap-Ph complemented this mutation, unambiguously showing the essential role of this peptide for bacteriocin production. Another sakacin A producer, LTH1174, had a similar response to temperature and exogenously added Sap-Ph.

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Keyword(s): BU, bacteriocin unit , HPK, histidine protein kinase , IF, induction factor , IU, induction unit , peptide pheromones , RR, response regulator , temperature-sensitive bacteriocin production and three-component regulatory systems
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2000-09-01
2025-05-20
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/content/journal/micro/10.1099/00221287-146-9-2155
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The synthesis of the bacteriocin sakacin A is a temperature-sensitive process regulated by a pheromone peptide through a three-component regulatory system
Microbiology 146, 2155 (2000); https://doi.org/10.1099/00221287-146-9-2155
/content/journal/micro/10.1099/00221287-146-9-2155
/content/journal/micro/10.1099/00221287-146-9-2155
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