1887

Abstract

Sakacin A is a bacteriocin produced by Lb706. The gene cluster () encompasses a regulatory unit composed of three consecutive genes, and . encode a histidine protein kinase and a response regulator, while encodes the putative precursor of a 23-amino-acid cationic peptide (termed Sap-Ph). The authors show that Sap-Ph serves as a pheromone regulating bacteriocin production. Lb706 produced bacteriocin when the growth temperature was kept at 25 or 30 °C, but production was reduced or absent at higher temperatures (335–35 °C). Production was restored by lowering the growth temperature to 30 °C, but at temperatures of 33–34 °C also by adding exogenous Sap-Ph to the growth medium. A knock-out mutation in abolished sakacin A production. Exogenously added Sap-Ph complemented this mutation, unambiguously showing the essential role of this peptide for bacteriocin production. Another sakacin A producer, LTH1174, had a similar response to temperature and exogenously added Sap-Ph.

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2000-09-01
2020-04-09
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