1887

Abstract

The pectinolytic enterobacterium 3937 causes a systemic disease in its natural host, the African violet ( ). It produces two structurally unrelated siderophores, chrysobactin and achromobactin. Chrysobactin makes a large contribution to invasive growth of the bacterium in its host. Insertion mutants of a chrysobactin-defective strain were constructed and screened on the universal CAS-agar medium used for siderophore detection. A set of mutants affected in the production of achromobactin were identified. This paper describes a mutant affected in the transport of all the ferrisiderophores used by the bacterium as iron sources. Molecular analysis revealed that the insertion mutation disrupts the gene. The predicted TonB protein has a molecular mass of 27600 Da and shares 20–58% identity with the TonB proteins from 20 other bacterial species. The pathogenicity of the mutant was assessed by inoculation of African violets. The impairment in the spread of symptoms was similar in the mutant to that in chrysobactin-defective mutants. However, the pectinolytic activity, the major pathogenicity determinant in , appeared to be stimulated twofold in the mutant.

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2000-08-01
2020-09-25
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