@article{mbs:/content/journal/micro/10.1099/00221287-146-8-2027, author = "Mora, Diego and Fortina, Maria Grazia and Parini, Carlo and Daffonchio, Daniele and Manachini, Pier Luigi", title = "Genomic subpopulations within the species Pediococcus acidilactici detected by multilocus typing analysis: relationships between pediocin AcH/PA-1 producing and non-producing strainsThe GenBank accession numbers for the sequences determined in this work are given in Table 4T4.", journal= "Microbiology", year = "2000", volume = "146", number = "8", pages = "2027-2038", doi = "https://doi.org/10.1099/00221287-146-8-2027", url = "https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-146-8-2027", publisher = "Microbiology Society", issn = "1465-2080", type = "Journal Article", keywords = "Pediococcus acidilactici", keywords = "pediocin AcH/PA-1", keywords = "intraspecific genetic variability", keywords = "LAB, lactic acid bacteria", keywords = "multilocus typing", keywords = "RAPD, random amplified polymorphic DNA", abstract = "A high degree of genetic polymorphism among P. acidilactici strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic DNA (RAPD) fingerprint analysis performed by using a single primer pedA gene targeted in low-stringency amplification conditions. Restriction fragment length polymorphism of the rpoC, ldhD/L and mle genes, and a modified RAPD analysis, permitted the grouping of Pediococcus acidilactici strains in seven genotypes (I–VII). Genotypic results obtained by analysing housekeeping genes involved in the transcription/translation machinery and in primary metabolism were supported by phylogenetic analysis based on the partial 16S rDNA sequencing of a reference strain of each of the seven clusters obtained. Three of the seven genotypes detected showed relationships with pediocin AcH/PA-1 production and carbohydrate fermentation patterns: all pediocin-producing and sucrose-positive strains were grouped in genotype VII, melibiose-, sucrose- and raffinose-positive strains in genotype VI, and arabinose-positive strains in genotype V.", }