1887

Abstract

A high degree of genetic polymorphism among strains was highlighted by a multilocus typing approach analysing several housekeeping genes and by sampling the whole genome using random amplified polymorphic DNA (RAPD) fingerprint analysis performed by using a single primer gene targeted in low-stringency amplification conditions. Restriction fragment length polymorphism of the , / and genes, and a modified RAPD analysis, permitted the grouping of strains in seven genotypes (I–VII). Genotypic results obtained by analysing housekeeping genes involved in the transcription/translation machinery and in primary metabolism were supported by phylogenetic analysis based on the partial 16S rDNA sequencing of a reference strain of each of the seven clusters obtained. Three of the seven genotypes detected showed relationships with pediocin AcH/PA-1 production and carbohydrate fermentation patterns: all pediocin-producing and sucrose-positive strains were grouped in genotype VII, melibiose-, sucrose- and raffinose-positive strains in genotype VI, and arabinose-positive strains in genotype V.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-8-2027
2000-08-01
2021-10-24
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/8/1462027a.html?itemId=/content/journal/micro/10.1099/00221287-146-8-2027&mimeType=html&fmt=ahah

References

  1. Back W., Stackebrandt E. 1978; DNS/DNS-Homologiestudien innerhalb der Gattung Pediococcus. Arch Microbiol 118:79–85 [CrossRef]
    [Google Scholar]
  2. Bhunia A. K., Johnson M. C., Ray B. 1988; Purification, characterization and antimicrobial spectrum of a bacteriocin produced by Pediococcus acidilactici. J Appl Bacteriol 65:261–268 [CrossRef]
    [Google Scholar]
  3. Bhunia A. K., Johnson M. C., Ray B., Kalchayan N. 1991; Mode of action of pediocin AcH from Pediococcus acidilactici H on sensitive bacterial strains. J Appl Bacteriol 70:25–33 [CrossRef]
    [Google Scholar]
  4. Cai Y., Kumai S., Ogawa M., Benno Y., Nakase T. 1999; Characterization and identification of Pediococcus species from forage crops and their application for silage preparation. Appl Environ Microbiol 65:2901–2906
    [Google Scholar]
  5. Chen Y., Ludescher R. D., Montville T. J. 1997; Electrostatic interactions, but not the YGNGV consensus motif, govern the binding of pediocin PA-1 and its fragments to phospholipid vesicles. Appl Environ Microbiol 63:4770–4777
    [Google Scholar]
  6. Chikindas M. L., Venema K., Ledeboer A. M., Venema G., Kok J. 1995; Expression of lactococcin A and pediocin PA-1 in heterologous hosts. Lett Appl Microbiol 21:183–189 [CrossRef]
    [Google Scholar]
  7. Collins M. D., Williams A. M., Wallbanks S. 1990; The phylogeny of Aerococcus and Pediococcus as determined by 16S rRNA sequence analysis: description of Tetragenococcus gen. nov. FEMS Microbiol Lett 70:255–262
    [Google Scholar]
  8. Daba H., Pandian H. D. S., Gosselin J. F., Simard R. E., Huang J., Lacroix C. 1991; Detection and activity of a bacteriocin produced by Leuconostoc mesenteroides. Appl Environ Microbiol 57:3450–3455
    [Google Scholar]
  9. Dellaglio F., Trovatelli L. D., Sarra P. G. 1981; DNA–DNA homology among representative strains of the genus Pediococcus. Zbl Bakt Hyg Abt Orig C2140–150
    [Google Scholar]
  10. Eldar A., Goria M., Ghittino C., Zlotkin A., Bercovier H. 1999; Biodiversity of Lactobacillus garvieae strains isolated from fish in Europe, Asia and Australia. Appl Environ Microbiol 65:1005–1008
    [Google Scholar]
  11. Fimland G., Blingsmo O. R., Sletten K., Jung G., Nes I., Nissen-Meyer J. 1996; New biologically active hybrid bacteriocins constructed by combining regions from various pediocin-like bacteriocins: the C-terminal region is important for determining specificity. Appl Environ Microbiol 62:3313–3318
    [Google Scholar]
  12. Garmyn D., Ferain T., Bernard N., Hols P., Delplace B., Delcour J. 1995a; Pediococcus acidilactici ldhD gene: cloning, nucleotide sequence, and transcriptional analysis. J Bacteriol 177:3427–3437
    [Google Scholar]
  13. Garmyn D., Ferain T., Bernard N., Hols P., Delcour J. 1995b; Cloning, nucleotide sequence, and transcriptional analysis of the Pediococcus acidilactici l-(+)-lactate dehydrogenase gene. Appl Environ Microbiol 61:266–272
    [Google Scholar]
  14. Garvie E. I. 1986; Genus Pediococcus. In Bergey’s Manual of Systematic Bacteriology pp. 1075Edited by Sneath P. H. A., Mair N. S., Sharpe M. E., Holt J. G. Baltimore: Williams & Wilkins;
    [Google Scholar]
  15. Groisillier A., Lonvaud-Funel A. 1999; Comparison of partial malolactic enzyme gene sequences for phylogenetic analysis of some lactic acid bacteria species and relationships with the malic enzyme. Int J Syst Bacteriol 49:1417–1428 [CrossRef]
    [Google Scholar]
  16. Horn N., Martı́nez M. I., Hernández P. E., Gasson M. J., Rodrı́guez J. M., Dodd H. M. 1998; Production of pediocin PA-1 by Lactococcus lactis using the lactococcin A secretory apparatus. Appl Environ Microbiol 64:818–823
    [Google Scholar]
  17. Lane D. J. 1991; 16S/23S rRNA sequencing. In Nucleic Acid Techniques in Bacterial Systematics pp. 115–175Edited by Stackebrandt E., Goodfellow M. Chichester: Wiley;
    [Google Scholar]
  18. Leisner J. J., Pot B., Christensen H., Rusul G., Olsen J. E., Wee B. W., Muhamad K., Ghazali H. M. 1999; Identification of lactic acid bacteria from chili bo, a Malaysian food ingredient. Appl Environ Microbiol 65:599–605
    [Google Scholar]
  19. Luchansky J. B., Glass K. A., Harsono K. D . 8 other authors 1992; Genomic analysis of Pediococcus starter cultures used to control Listeria monocytogenes in turkey summer sausage. Appl Environ Microbiol 58:3053–3059
    [Google Scholar]
  20. Maiden M. C. J., Bygraves J. A., Feil E.10 other authors 1998; Multilocus sequence typing: a portable approach to the identification of clones within populations of pathogenic microorganisms. Proc Natl Acad Sci USA 95:3140–3145 [CrossRef]
    [Google Scholar]
  21. de Mann J. C., Rogosa M., Sharpe M. E. 1960; A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130–135 [CrossRef]
    [Google Scholar]
  22. Marugg J. D., Gonzalez C. F., Kunka B. S., Lebeboer A. M., Pucci M. J., Toonen M. Y., Walker S. A., Zoetmudler L. C. M., Vanderbergh P. A. 1992; Cloning, expression, and nucleotide sequence of genes involved in the production of pediocin PA-1, a bacteriocin from Pediococcus acidilactici PAC1.0. Appl Environ Microbiol 58:2360–2367
    [Google Scholar]
  23. Miller K. W., Schamber R., Osmanagaoglu O., Ray B. 1998; Isolation and characterization of pediocin AcH chimeric protein mutants with altered bactericidal activity. Appl Environ Microbiol 64:1997–2005
    [Google Scholar]
  24. Mora D., Fortina M. G., Parini C., Manachini P. L. 1997; Identification of Pediococcus acidilactici and Pediococcus pentosaceus based on 16S rRNA and ldhD gene-targeted multiplex PCR analysis. FEMS Microbiol Lett 151:231–236 [CrossRef]
    [Google Scholar]
  25. Mora D., Parini C., Fortina M. G., Manachini P. L. 1998; Discrimination among pediocin AcH/PA-1 producer strains by comparison of pedB and pedD amplified genes and by multiplex PCR assay. Syst Appl Microbiol 21:454–460 [CrossRef]
    [Google Scholar]
  26. Morse R., Collins M. D., Balsdon J. T., Wallbanks S., Richardson P. T. 1996; Nucleotide sequence of part of the rpoC gene encoding the β′ subunit of DNA-dependent RNA polymerase from some Gram-positive bacteria and comparative amino acids sequence analysis. Syst Appl Microbiol 19:150–157 [CrossRef]
    [Google Scholar]
  27. Motlagh A. M., Holla S., Johnson M. C., Ray B., Field R. A. 1992; Inhibition of Listeria spp. in sterile food system by pediocin AcH, a bacteriocin produced by Pediococcus acidilactici H. J Food Prot 55:337–343
    [Google Scholar]
  28. Motlagh A., Bukhtiyarova M., Ray B. 1994; Complete nucleotide sequence of pSMB74, a plasmid encoding the production of pediocin AcH in Pediococcus acidilactici. Lett Appl Microbiol 18:305–312 [CrossRef]
    [Google Scholar]
  29. Nakagawa A., Kitahara K. 1959; Taxonomic studies on the genus Pediococcus. J Gen Appl Microbiol 5:95–126 [CrossRef]
    [Google Scholar]
  30. Okereke A., Montville T. J. 1991; Bacteriocin inhibition of Clostridium botulinum spores by lactic acid bacteria. J Food Prot 1:349–353
    [Google Scholar]
  31. Palys T., Nakamura L. K., Cohan F. M. 1997; Discovery and classification of ecological diversity in the bacterial world: the role of DNA sequence data. Int J Syst Bacteriol 47:1145–1156 [CrossRef]
    [Google Scholar]
  32. Pearson W. R. 1990; Rapid and sensitive sequence comparison with fastp and fast a. Methods Enzymol 183:63–98
    [Google Scholar]
  33. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence analysis. Proc Natl Acad Sci USA 85:2444–2448 [CrossRef]
    [Google Scholar]
  34. Pucci M. J., Vedamuthu E. R., Kunka B. S., Vandenbergh P. A. 1988; Inhibition of Listeria monocytogenes by using bacteriocin PA-1 produced by Pediococcus acidilactici PAC1.0. Appl Environ Microbiol 54:2349–2353
    [Google Scholar]
  35. Ray B. 1996; Characteristics and applications of pediocin(s) of Pediococcus acidilactici: pediocin PA-1/AcH. In Lactic Acid Bacteria: Current Advances in Metabolism, Genetics and Applications pp. 155–203Edited by Bozoglu T. F., Ray B. Berlin & Heidelberg: Springer;
    [Google Scholar]
  36. Rohlf F. J. 1987 ntsys-pc: numerical taxonomy and multivariate analysis system for IBM PC microcomputer (and compatibles), version 1.30 New York: Applied Biostatistics;
    [Google Scholar]
  37. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  38. Schoeman H., Vivier M. A., Du Toit M., Dicks L. M. T., Pretorius I. S. 1999; The development of bactericidal yeast strains by expressing the Pediococcus acidilactici pediocin gene (pedA) in Saccharomyces cerevisiae. Yeast 15:647–656 [CrossRef]
    [Google Scholar]
  39. Sharpe E. 1962; Taxonomy of the lactobacilli. Dairy Sci Abstr 24:109–118
    [Google Scholar]
  40. Sneath P. H. A., Sokal R. R. 1973 Numerical Taxonomy: the Principles and Practice of Numerical Classification San Francisco: W. H. Freeman;
    [Google Scholar]
  41. Stiles M. E. 1996; Biopreservation by lactic acid bacteria. Antonie Leeuwenhoek 70:331–345 [CrossRef]
    [Google Scholar]
  42. Urbach E., Daniels B., Salama M. S., Sandine W. E., Giovannoni S. T. 1997; The ldh phylogeny for environmental isolates of Lactococcus lactis is consistent with rRNA genotypes but not with phenotypes. Appl Environ Microbiol 63:694–702
    [Google Scholar]
  43. Venema K., Kok J., Marugg J. D., Toonen M. Y., Ledeboer A. M., Venema G., Chikindas M. L. 1995; Functional analysis of the pediocin operon of Pediococcus acidilactici PAC1.0: PedB is the immunity protein and PedD is the precursor processing enzyme. Mol Microbiol 17:515–522 [CrossRef]
    [Google Scholar]
  44. Welsh J., McClelland M. 1990; Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res 18:7213–7218 [CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-8-2027
Loading
/content/journal/micro/10.1099/00221287-146-8-2027
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error