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Volume 146, Issue 8

Fruiting body development in : regulated expression of two galectins secreted by a non-classical pathway

The GenBank accession number for the sequence reported in this paper is AF130360

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Abstract

Fruiting body formation in the basidiomycete is a developmental process that occurs as a response of the mycelium to external stimuli. First, localized, highly branched hyphal structures (knots) are formed as a reaction to nutritional depletion. Hyphal-knot formation is repressed by light; however, light signals are essential for the development of the hyphal knot into an embryonic fruiting body (primordium) as well as karyogamy, meiosis and fruiting body maturation. The role of the different environmental signals in the initial phases of fruiting body development was analysed. It was observed that two fungal galectins, Cgl1 and Cgl2, are differentially regulated during fruiting body formation. expression initiated in early stages of fruiting body development (hyphal knot formation) and was maintained until maturation of the fruiting body, whereas was specifically expressed in primordia and mature fruiting bodies. Immunofluorescence and immuno-electron microscopy studies detected galectins within specific fruiting body tissues. They localized in the extracellular matrix and the cell wall but also in membrane-bound bodies in the cytoplasm. Heterologous expression of Cgl2 in indicated that secretion of this protein occurred independently of the classical secretory pathway.

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Keyword(s): basidiomycete , Coprinus , ECM, extracellular matrix , fruiting body , galectin and secretion
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2000-08-01
2024-04-25
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Fruiting body development in Coprinus cinereus: regulated expression of two galectins secreted by a non-classical pathwayThe GenBank accession number for the sequence reported in this paper is AF130360
Microbiology 146, 1841 (2000); https://doi.org/10.1099/00221287-146-8-1841
/content/journal/micro/10.1099/00221287-146-8-1841
/content/journal/micro/10.1099/00221287-146-8-1841
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