1887

Abstract

The yeast gene encodes glucosamine-6-phosphate acetyltransferase which catalyses the reaction of glucosamine 6-phosphate with acetyl-CoA to form -acetylglucosamine 6-phosphate, a fundamental precursor in UDP--acetylglucosamine biosynthesis. mutants lacking were viable in the presence of -acetylglucosamine. To confirm the physiological importance of , the virulence of a Δ null mutant was examined in a mouse model of candidiasis. When injected intravenously into mice, the virulence of the Δ null mutant was significantly attenuated. The reduced virulence appeared to be the result of rapid clearance from host tissue. These data suggest that is required for survival of the fungus in host animals, probably because an insufficient level of -acetylglucosamine is available from the host tissues.

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2000-07-01
2024-04-19
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References

  1. Alani E., Cao L., Kleckner N. 1987; A method for gene disruption that allows repeated use of URA3 selection in the construction of multiply disrupted yeast strains. Genetics 116:541–545 [CrossRef]
    [Google Scholar]
  2. Biswas M., Singh B., Datta A. 1979; Induction of N-acetylmannosamine catabolic pathway in yeast. Biochim Biophys Acta 585:535–542 [CrossRef]
    [Google Scholar]
  3. Boles E., Liebetrau W., Hofmann M., Zimmermann F. K. 1994; A family of hexosephosphate mutases in Saccharomyces cerevisiae. Eur J Biochem 220:83–96 [CrossRef]
    [Google Scholar]
  4. Cabib E., Roberts R., Bowers B. 1982; Synthesis of the yeast cell wall and its regulation. Annu Rev Biochem 51:763–793 [CrossRef]
    [Google Scholar]
  5. Datta A., Ganesan K., Natarajan K. 1989; Current trends in Candida albicans research. Adv Microb Physiol 30:53–88
    [Google Scholar]
  6. Fonzi W. A., Irwin M. Y. 1993; Isogenic strain construction and gene mapping in Candida albicans. Genetics 134:717–728
    [Google Scholar]
  7. Gehring A. M., Lees W. J., Mindiola D. J., Walsh C. T., Brown E. D. 1996; Acetyltransfer precedes uridylyltransfer in the formation of UDP-N-acetylglucosamine in separable active sites of the bifunctional GlmU protein of Escherichia coli. Biochemistry 35:579–585 [CrossRef]
    [Google Scholar]
  8. Gopal P., Sullivan P. A., Shepherd M. G. 1982; Enzymes of N-acetylglucosamine metabolism during germ-tube formation in Candida albicans. J Gen Microbiol 128:2319–2326
    [Google Scholar]
  9. Gow N. A. R., Gooday G. W. 1987; Cytological aspects of dimorphism in Candida albicans. Crit Rev Microbiol 15:73–78 [CrossRef]
    [Google Scholar]
  10. Herscovics A., Orlean P. 1993; Glycoprotein biosynthesis in yeast. FASEB J 7:540–550
    [Google Scholar]
  11. Hofmann M., Boles E., Zimmermann F. K. 1994; Characterization of the essential yeast gene encoding N-acetylglucosamine-phosphate mutase. Eur J Biochem 221:741–747 [CrossRef]
    [Google Scholar]
  12. Holtje J. V., Schwartz U. 1985 Molecular Cytology of Escherichia coli pp. 77–119Edited by Nanninga N. New York: Academic Press;
    [Google Scholar]
  13. Ito H., Fukuda Y., Murata K., Kimura A. 1983; Transformation of intact yeast cells treated with alkali cations. J Bacteriol 153:163–168
    [Google Scholar]
  14. Kasahara S., Yamada H., Mio T., Shiratori Y., Miyamoto C., Yabe T., Nakajima T., Ichishima E., Furuichi Y. 1994; Cloning of the Saccharomyces cerevisiae gene whose overexpression overcomes the effects of HM-1 killer toxin, which inhibits β-glucan synthesis. J. Bacteriol 176:1488–1499
    [Google Scholar]
  15. Lundblad V. 1992; Saccharomyces cerevisiae. In Current Protocols in Molecular Biology pp. 13.1–13.13Edited by Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. New York: Wiley Interscience;
    [Google Scholar]
  16. Mio T., Yabe T., Arisawa M., Yamada-Okabe H. 1998; The eukaryotic UDP-N-acetylglucosamine pyrophosphorylases. Gene cloning, protein expression, and catalytic mechanism. J Biol Chem 273:14392–14397 [CrossRef]
    [Google Scholar]
  17. Mio T., Yamada-Okabe T., Arisawa M., Yamada-Okabe H. 1999; Saccharomyces cerevisiae GNA1, an essential gene encoding a novel acetyltransferase involved in UDP-N-acetylglucosamine synthesis. J Biol Chem 274:424–429 [CrossRef]
    [Google Scholar]
  18. Odds F. C. 1987; Candida infection: an overview. Crit Rev Microbiol 15:1–5 [CrossRef]
    [Google Scholar]
  19. Odds F. C. 1994; Candida species and virulence. ASM News 60:313–318
    [Google Scholar]
  20. Park J. T. 1987 Escherichia coli and Salmonella typhimurinum: Cellular and Molecular Biology pp. 663–671Edited by Neidhardt F. C.others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  21. Raetz C. R. H. 1987 Escherichia coli and Salmonella typhimurinum: Cellular and Molecular Biology pp. 498–503Edited by Neidhardt F. C.others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  22. Riddles P. W., Blakeley R. L., Zerner B. 1983; Reassessment of Ellman’s reagent. Methods Enzymol 91:49–60
    [Google Scholar]
  23. Shepherd M. G. 1985; Candida albicans: biology, genetics, and pathogenicity. Annu Rev Microbiol 39:579–614 [CrossRef]
    [Google Scholar]
  24. Singh B., Datta A. 1979; Induction of N-acetylglucosamine-catabolic pathway in spheroplasts of Candida albicans. Biochem J 178:427–431
    [Google Scholar]
  25. Smith R. J., Milewski S., Brown A. J., Gooday G. W. 1996; Isolation and characterization of the GFA1 gene encoding the glutamine:fructose-6-phosphate amidotransferase of Candida albicans. J Bacteriol 178:2320–2327
    [Google Scholar]
  26. Watzele G., Tanner W. 1989; Cloning of the glutamine:fructose-6-phosphate amidotransferase gene from yeast. Pheromonal regulation of its transcription. J Biol Chem 264:8753–8758
    [Google Scholar]
  27. Whelan W. L., Ballou C. E. 1975; Sporulation in d-glucosamine auxotrophs of Saccharomyces cerevisiae: meiosis with defective ascospore wall formation. J Bacteriol 125:1545–1557
    [Google Scholar]
  28. Yamada-Okabe T., Shimmi O., Doi R., Mizumoto K., Arisawa M., Yamada-Okabe H. 1996; Isolation of the mRNA-capping enzyme and ferric-reductase-related genes from Candida albicans. Microbiology 142:2515–2523 [CrossRef]
    [Google Scholar]
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