1887

Abstract

In contrast to what happens in and , synthesis of periplasmic cyclic 1,2-β-glucan in spp. was not inhibited when bacteria were grown in media of high osmolarity. Studies performed with crude membrane preparations showed that cyclic 1,2-β-glucan synthetase of spp. was not inhibited by 05 M KCl or potassium glutamate; concentrations that completely inhibit the osmosensitive enzymes of A348 or 102F34, respectively encoded by the or genes. The cyclic 1,2-β-glucan synthetase gene () was introduced into A1011 and GRT21s mutants. Synthesis of cyclic 1,2-β-glucan by the recombinant strains was not inhibited when grown in media of high osmolarity (025 M NaCl or 05 M mannitol). On the other hand, when the cyclic 1,2-β-glucan synthetase gene was introduced into the GRT21s mutant, the recombinant strain displayed marked inhibition of cyclic 1,2-β-glucan synthesis when grown in high-osmolarity media. However, the same gene introduced into a mutant background resulted in no inhibition of glucan synthesis at high osmolarity. studies with crude membranes isolated from recombinant strains revealed that cyclic 1,2-β-glucan synthetase was not inhibited by high concentrations of KCl or potassium glutamate even when expressed in or backgrounds. It was concluded that the lack of effect of high osmolarity on 1,2-β-glucan synthesis in is due to two convergent mechanisms: a) the presence of a cyclic 1,2-β-glucan synthetase that is not affected by concentrations of solutes such as KCl or potassium glutamate and b) either the possible accumulation of compatible solutes that might protect the enzyme from the inhibition by potassium glutamate or the accumulation of other osmolytes that do not affect the 1,2-β-glucan synthetase.

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2000-07-01
2020-04-04
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