1887

Abstract

The gene encoding acetyl xylan esterase () was identified and characterized. The gene was expressed and the recombinant enzyme produced in was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) purified from . The AXE primary structure was 76% identical to the cephalosporin C deacetylase of , and 40% to two recently identified AXEs from and . These four proteins are of similar size and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.

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2000-07-01
2024-12-04
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