1887

Abstract

The gene encoding acetyl xylan esterase () was identified and characterized. The gene was expressed and the recombinant enzyme produced in was purified and characterized. The recombinant enzyme displayed similar properties to the acetyl xylan esterase (AXE) purified from . The AXE primary structure was 76% identical to the cephalosporin C deacetylase of , and 40% to two recently identified AXEs from and . These four proteins are of similar size and represent a new family of esterases having a broad substrate specificity. The recombinant AXE was demonstrated to have activity on several acetylated substrates, including on cephalosporin C.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-7-1585
2000-07-01
2019-12-15
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/7/1461585a.html?itemId=/content/journal/micro/10.1099/00221287-146-7-1585&mimeType=html&fmt=ahah

References

  1. Ausubel, F. M., Brent, R., Kingstone, R. E., Moore, D. D., Seidman, J. G., Smith, J. A. & Struhl, K. (1988). Current Protocols in Molecular Biology. New York: Greene Publishing.
  2. Biely, P. ( 1985; ). Microbial xylanolytic system. Trends Biotechnol 3, 286-290.[CrossRef]
    [Google Scholar]
  3. Biely, P., MacKenzie, C. R., Puls, J. & Schneider, H. ( 1986; ). Cooperativity of esterases and xylanases in the enzymatic degradation of acetyl xylan. BioTechnology 4, 731-733.[CrossRef]
    [Google Scholar]
  4. Birnboim, H. C. ( 1983; ). A rapid alkaline extraction method for the isolation of plasmid DNA. Methods Enzymol 100, 243-255.
    [Google Scholar]
  5. Brenner, S. ( 1988; ). The molecular evolution of genes and proteins: a tale of two serines. Nature 334, 528-530.[CrossRef]
    [Google Scholar]
  6. Bullock, W. O., Fernandez, J. M. & Short, J. M. ( 1987; ). XL-1 Blue: a high efficiency plasmid transforming recA Escherichia coli strain with β-galactosidase selection. Biotechniques 5, 376-382.
    [Google Scholar]
  7. Christov, L. P. & Prior, B. A. ( 1993; ). Esterases of xylan-degrading microorganisms: production, properties, and significance. Enzyme Microb Technol 15, 460-475.[CrossRef]
    [Google Scholar]
  8. Coutinho, P. M. & Henrissat, B. (1999). Carbohydrate-Active Enzyme server. http://afmb.cnrs-mrs.fr/~pedro/CAZY/db.html.
  9. Degrassi, G., Okeke, B. C., Bruschi, C. V. & Venturi, V. ( 1998; ). Purification and characterization of an acetyl xylan esterase from Bacillus pumilus. Appl Environ Microbiol 64, 789-792.
    [Google Scholar]
  10. Hanahan, D. ( 1983; ). Studies on transformation of Escherichia coli with plasmids. J Mol Biol 166, 557-580.[CrossRef]
    [Google Scholar]
  11. Lindberg, B., Rossel, K. G. & Svensson, S. ( 1973; ). Position of the O-acetyl groups in birch xylan. Sven Papperstidn 76, 30-32.
    [Google Scholar]
  12. Lorenz, W. W. & Wiegel, J. ( 1997; ). Isolation, analysis, and expression of two genes from Thermoanaerobacterium sp. strain JW/SL YS485: a β-xylosidase and a novel acetyl xylan esterase with cephalosporin C deacetylase activity. J Bacteriol 179, 5436-5441.
    [Google Scholar]
  13. Maniatis, T., Fritsch, E. F. & Sambrook, J. (1982). Molecular Cloning: a Laboratory Manual. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  14. Mitsushima, K., Takimoto, A., Sonoyama, T. & Yagi, S. ( 1995; ). Gene cloning, nucleotide sequence, and expression of a cephalosporin-C deacetylase from Bacillus subtilis. Appl Environ Microbiol 61, 2224-2229.
    [Google Scholar]
  15. Moriyama, H., Fukusaki, E., Cabrera Crespo, J., Shinmyo, A. & Okada, H. ( 1987; ). Structure and expression of genes coding for xylan-degrading enzymes of Bacillus pumilus. Eur J Biochem 166, 539-545.[CrossRef]
    [Google Scholar]
  16. Panbangred, W., Kondo, T., Negoro, S., Shinmyo, A. & Okada, H. ( 1983; ). Molecular cloning of the genes for xylan degradation of Bacillus pumilus and their expression in Escherichia coli. Mol Gen Genet 192, 335-341.[CrossRef]
    [Google Scholar]
  17. Panbangred, W., Kawaguchi, O., Tomita, T., Shinmyo, A. & Okada, H. ( 1984; ). Isolation of two β-xylosidase genes of Bacillus pumilus and comparison of their gene products. Eur J Biochem 138, 267-273.[CrossRef]
    [Google Scholar]
  18. Poutanen, K. & Sundberg, M. ( 1988; ). An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylans. Appl Microbiol Biotechnol 28, 419-424.[CrossRef]
    [Google Scholar]
  19. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989). Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory.
  20. Sanger, F., Nicklen, S. & Coulson, A. R. ( 1977; ). DNA sequencing with chain terminating inhibitors. Proc Natl Acad Sci USA 74, 5463-5467.[CrossRef]
    [Google Scholar]
  21. Simonen, M. & Palva, I. ( 1993; ). Protein secretion in Bacillus species. Microbiol Rev 57, 109-137.
    [Google Scholar]
  22. Takimoto, A., Mitsushima, K., Yagi, S. & Sonoyama, T. ( 1994; ). Purification, characterization and partial amino acid sequences of a novel cephalosporin-C deacetylase from Bacillus subtilis. J Ferment Bioeng 77, 17-22.[CrossRef]
    [Google Scholar]
  23. Whistler, R. L. & Richards, E. L. ( 1970; ). Hemicelluloses. In The Carbohydrates – Chemistry and Biochemistry, pp. 447-469. Edited by W. Pigman & D. Horton. New York: Academic Press.
  24. Yanisch-Perron, C., Vieira, J. & Messing, J. ( 1985; ). Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33, 103-119.[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-7-1585
Loading
/content/journal/micro/10.1099/00221287-146-7-1585
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error