1887

Abstract

Essential two-component systems are now being identified in bacteria. The gene encoding a response regulator, and its orthologue in , were reported recently to be essential for cell growth, although genes under their control have yet to be identified. The essential nature of the regulator gene and its cognate kinase gene, , in was also noted during the course of construction of a knockout mutant bank of newly identified genes in the genome sequence project. It was found that could be deleted in the presence of an active form of the YycF protein, thereby suggesting direct interaction between YycG and YycF. Production of mini-cells and reduction in cell length occurred when the YycF regulator was overproduced in . These observations led to the finding that YycF overproduction up-regulated the expression from the P1 promoter of the cell division operon, . In addition, the YycF protein binds to the P1 promoter region . These results clearly indicate that the essential two-component regulatory system encoded by and genes has the potential to modulate expression of the operon in .

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2000-07-01
2019-09-23
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