1887

Abstract

Transfer of VanB-type resistance to glycopeptides among enterococci has been reported to be associated with the movement of large chromosomal genetic elements or of plasmids. The authors report the characterization of the 34 kb transposon Tn borne by a plasmid related to pAD1 and conferring vancomycin resistance in clinical isolates of spp. Tn contained 30 ORFs and appeared to be organized like the Tn family of conjugative transposons into three functional regions: (i) the right end, implicated in the excision–integration process; (ii) the central part, in which the operon replaces the (M) gene; and (iii) the left extremity, in which eight of the 18 ORFs could be implicated in the conjugative transfer.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-6-1481
2000-06-01
2024-11-10
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/6/1461481a.html?itemId=/content/journal/micro/10.1099/00221287-146-6-1481&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res 25:3389–3402 [CrossRef]
    [Google Scholar]
  2. Arthur M., Reynolds P. E., Courvalin P. 1996; Glycopeptide resistance in enterococci. Trends Microbiol 4:401–407 [CrossRef]
    [Google Scholar]
  3. Ausubel F. M., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. 1992; Preparation and analysis of DNA. Current Protocols in Molecular Biology2–33 New York: Wiley;
    [Google Scholar]
  4. Beloin C., Ayora S., Exley R., Hirschbein L., Ogasawara N., Kasahara Y., Alonso J. C., Le Hégarat F. 1997; Characterization of an lrp-like (lprC) gene from Bacillus subtilis. Mol Gen Genet 256:63–71 [CrossRef]
    [Google Scholar]
  5. Berg T., Firth N., Apisiridej S., Hettiaratchi A., Leelaporn A., Skurray R. A. 1998; Complete nucleotide sequence of pSK41: evolution of staphylococcal conjugative multiresistance plasmids. J Bacteriol 180:4350–4359
    [Google Scholar]
  6. Buchrieser C., Rusniok C., Frangeul L., Couve E., Billault A., Kunst F., Carniel E., Glaser P. 1999; The 102-kilobase pgm locus of Yersinia pestis: sequence analysis and comparison of selected regions among different Yersinia pestis and Yersinia pseudotuberculosis strains. Infect Immun 67:4851–4861
    [Google Scholar]
  7. Carias L. L., Rudin S. D., Donskey C. J., Rice L. B. 1998; Genetic linkage and cotransfer of a novel, vanB-containing transposon (Tn5382) and a low-affinity penicillin-binding protein 5 gene in a clinical vancomycin-resistant Enterococcus faecium isolate. J Bacteriol 180:4426–4434
    [Google Scholar]
  8. Celli J., Trieu-Cuot P. 1998; Circularization of Tn916 is required for expression of the transposon-encoded transfer functions: characterization of long tetracycline-inducible transcripts reading through the attachment site. Mol Microbiol 28:103–117
    [Google Scholar]
  9. Clewell D. B. 1993; Bacterial sex pheromone-induced plasmid transfer. Cell 73:9–12 [CrossRef]
    [Google Scholar]
  10. Clewell D. B., Flannagan S. E., Jaworski D. D. 1995; Unconstrained bacterial promiscuity: the Tn916-Tn1545 family of conjugative transposons. Trends Microbiol 3:229–236 [CrossRef]
    [Google Scholar]
  11. Courvalin P., Carlier C. 1986; Transposable multiple antibiotic resistance in Streptococcus pneumoniae. Mol Gen Genet 205:291–297 [CrossRef]
    [Google Scholar]
  12. Dahl K. H., Simonsen G. S., Olsvik O., Sundsfjord A. 1999; Heterogeneity in the vanB gene cluster of genomically diverse clinical strains of vancomycin-resistant enterococci. Antimicrob Agents Chemother 43:1105–1110
    [Google Scholar]
  13. Devriese L. A., Pot B., Collins M. D. 1993; Phenotypic identification of the genus Enterococcus and differentiation of phylogenetically distinct enterococcal species and species groups. J Appl Bacteriol 75:399–408 [CrossRef]
    [Google Scholar]
  14. Evers S. 1995 Genetic characterization of VanB-type glycopeptide resistance in Enterococcus faecalis V583 PhD thesis Technischen Universität München;
    [Google Scholar]
  15. Ewing B., Green P. 1998; Base-calling of automated sequencer traces using phred. II. Error probabilities. Genome Res 8:186–194
    [Google Scholar]
  16. Ewing B., Hillier L., Wendl M. C., Green P. 1998; Base-calling of automated sequencer traces using phred. I. Accuracy assessment. Genome Res 8:175–185 [CrossRef]
    [Google Scholar]
  17. Fines M., Périchon B., Reynolds P. E., Sahm D. F., Courvalin P. 1999; VanE, a new type of acquired glycopeptide resistance in Enterococcus faecalis BM4405. Antimicrob Agents Chemother 43:2161–2164
    [Google Scholar]
  18. Flannagan S. E., Zitzow L. A., Su Y. A., Clewell D. B. 1994; Nucleotide sequence of the 18-kb conjugative transposon Tn916 from Enterococcus faecalis. Plasmid 32:350–354 [CrossRef]
    [Google Scholar]
  19. Gold H. S., Ünal S., Cercenado E., Thauvin-Eliopoulos C., Eliopoulos G. M., Wennersten C. B., Moellering R. C. Jr 1993; A gene conferring resistance to vancomycin but not teicoplanin in isolates of Enterococcus faecalis and Enterococcus faecium demonstrates homology with vanB, vanA, and vanC genes of enterococci. Antimicrob Agents Chemother 37:1604–1609 [CrossRef]
    [Google Scholar]
  20. Gordon D., Abajian C., Green C. 1998; Consed: a graphical tool for sequence finishing. Genome Res 8:195–202 [CrossRef]
    [Google Scholar]
  21. Jacob A. E., Hobbs S. J. 1974; Conjugal transfer of plasmid-borne multiple antibiotic resistance in Streptococcus faecalis var. zymogenes. J Bacteriol 117:360–372
    [Google Scholar]
  22. Manganelli R., Romano L., Ricci S., Zazzi M., Pozzi G. 1995; Dosage of Tn916 circular intermediates in Enterococcus faecalis. Plasmid 34:48–57 [CrossRef]
    [Google Scholar]
  23. Morton T. M., Eaton D. M., Johnston J. L., Archer G. L. 1993; DNA sequence and units of transcription of the conjugative transfer gene complex (trs) of Staphylococcus aureus plasmid pGO1. J Bacteriol 175:4436–4447
    [Google Scholar]
  24. Murray B. E. 1990; The life and times of the Enterococcus. Clin Microbiol Rev 3:46–65
    [Google Scholar]
  25. Ozawa Y., Tanimoto K., Fujimoto S., Tomita H., Ike Y. 1997; Cloning and genetic analysis of the UV resistance determinant (uvr) encoded on the Enterococcus faecalis pheromone-responsive conjugative plasmid pAD1. J Bacteriol 179:7468–7475
    [Google Scholar]
  26. Pearson W. R., Lipman D. J. 1988; Improved tools for biological sequence comparison. Proc Natl Acad Sci USA 85:2444–2448 [CrossRef]
    [Google Scholar]
  27. Périchon B., Reynolds P. E., Courvalin P. 1997; VanD-type glycopeptide-resistant Enterococcus faecium BM4339. Antimicrob Agents Chemother 41:2016–2018
    [Google Scholar]
  28. Pontius L. T., Clewell D. B. 1992; Conjugative transfer of Enterococcus faecalis plasmid pAD1: nucleotide sequence and transcriptional fusion analysis of a region involved in positive regulation. J Bacteriol 174:3152–3160
    [Google Scholar]
  29. Poyart-Salmeron C., Trieu-Cuot P., Carlier C., Courvalin P. 1989; Molecular characterization of two proteins involved in the excision of the conjugative transposon Tn1545: homologies with other site-specific recombinases. EMBO J 8:2425–2433
    [Google Scholar]
  30. Poyart-Salmeron C., Trieu-Cuot P., Carlier C., Courvalin P. 1990; The integration–excision system of the conjugative transposon Tn1545 is structurally and functionally related to those of lambdoid phages. Mol Microbiol 4:1513–1521 [CrossRef]
    [Google Scholar]
  31. Projan S. J., Kornblum J., Moghazeh S. L., Edelman I., Gennaro M. L., Novick R. P. 1985; Comparative sequence and functional analysis of pT181 and pC221, cognate plasmid replicons from Staphylococcus aureus. Mol Gen Genet 199:452–464 [CrossRef]
    [Google Scholar]
  32. Quintiliani R. Jr, Courvalin P. 1994; Conjugal transfer of the vancomycin resistance determinant vanB between enterococci involves the movement of large genetic elements from chromosome to chromosome. FEMS Microbiol Lett 119:359–364 [CrossRef]
    [Google Scholar]
  33. Quintiliani R. Jr, Courvalin P. 1996; Characterization of Tn1547, a composite transposon flanked by the IS16 and IS256-like elements, that confers vancomycin resistance in Enterococcus faecalis BM4281. Gene 172:1–8 [CrossRef]
    [Google Scholar]
  34. Reynolds P. E. 1989; Structure, biochemistry and mechanism of action of glycopeptide antibiotics. Eur J Clin Microbiol Infect Dis 8:943–950 [CrossRef]
    [Google Scholar]
  35. Sambrook J., Fritsch E. F., Maniatis T. 1989 Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  36. Sanger F., Nicklen S., Coulson A. R. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74:5463–5467 [CrossRef]
    [Google Scholar]
  37. Siksnys V., Zareckaja N., Vaisvila R., Timinskas A., Stakenas P., Butkus V., Janulaitis A. 1994; CAATTG-specific restriction-modification MunI genes from Mycoplasma: sequence similarities between R-MunI and R-EcoRI. Gene 142:1–8 [CrossRef]
    [Google Scholar]
  38. Trieu-Cuot P., Poyart-Salmeron C., Carlier C., Courvalin P. 1993; Sequence requirements for target activity in site-specific recombination mediated by the Int protein of transposon Tn1545. Mol Microbiol 8:179–185 [CrossRef]
    [Google Scholar]
  39. Triglia T., Peterson M. G., Kemp D. J. 1988; A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences. Nucleic Acids Res 16:8186 [CrossRef]
    [Google Scholar]
  40. Vieira J., Messing J. 1982; The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers. Gene 19:259–268 [CrossRef]
    [Google Scholar]
  41. Woodford N., Jones B. L., Baccus Z., Ludlam H. A., Brown D. F. J. 1995a; Linkage of vancomycin and high-level gentamicin resistance genes on the same plasmid in a clinical isolate of Enterococcus faecalis. J Antimicrob Chemother 35:179–184 [CrossRef]
    [Google Scholar]
  42. Woodford N., Morrison D., Johnson A. P., Bateman A. C., Hastings J. G. M., Elliot T. S. J., Cookson B. 1995b; Plasmid-mediated VanB glycopeptide resistance in enterococci. Microb Drug Resist 1:235–240 [CrossRef]
    [Google Scholar]
  43. Yanisch-Perron C., Vieira J., Messing J. 1985; Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mp18 and pUC19 vectors. Gene 33:103–119 [CrossRef]
    [Google Scholar]
/content/journal/micro/10.1099/00221287-146-6-1481
Loading
/content/journal/micro/10.1099/00221287-146-6-1481
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error