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Volume 146, Issue 6

The gene encodes a DNA-binding protein that regulates daunorubicin production in by controlling expression of the pseudo response regulator gene Free

Abstract

The gene is located adjacent to and divergently transcribed from the response regulator gene, , that activates the transcription of the gene, which in turn activates transcription of the daunorubicin biosynthesis genes in . Gene disruption and replacement of produced the :: mutant strain and resulted in the complete loss of daunorubicin biosynthesis. Suppression of the :: mutation by the introduction of or on a plasmid suggested that DnrO is required for the transcription of , whose product is known to be required for expression. These conclusions were supported by the effects of the mutation on expression of , and , as viewed by fusions to each of these regulatory genes. DnrO was overexpressed in and the cell-free extract was used to conduct mobility shift DNA-binding assays. The results showed that DnrO binds specifically to the overlapping / promoter region. Thus, may regulate the expression of both the and genes.

  • Received:
  • Accepted:
  • Revised:
  • Published Online:
Keyword(s): anthracycline antitumour antibiotic , biosynthesis , DNR, daunorubicin , DXR, doxorubicin , HTH, helix–turn–helix , melanin gene fusions , RHO, ϵ-rhodomycinone and transcriptional mapping
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2000-06-01
2024-04-19
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The dnrO gene encodes a DNA-binding protein that regulates daunorubicin production in Streptomyces peucetius by controlling expression of the dnrN pseudo response regulator gene
Microbiology 146, 1457 (2000); https://doi.org/10.1099/00221287-146-6-1457
/content/journal/micro/10.1099/00221287-146-6-1457
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