1887

Abstract

The gene is located adjacent to and divergently transcribed from the response regulator gene, , that activates the transcription of the gene, which in turn activates transcription of the daunorubicin biosynthesis genes in . Gene disruption and replacement of produced the :: mutant strain and resulted in the complete loss of daunorubicin biosynthesis. Suppression of the :: mutation by the introduction of or on a plasmid suggested that DnrO is required for the transcription of , whose product is known to be required for expression. These conclusions were supported by the effects of the mutation on expression of , and , as viewed by fusions to each of these regulatory genes. DnrO was overexpressed in and the cell-free extract was used to conduct mobility shift DNA-binding assays. The results showed that DnrO binds specifically to the overlapping / promoter region. Thus, may regulate the expression of both the and genes.

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2000-06-01
2019-10-17
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