1887

Abstract

Under heat-stress conditions bacteria induce, among other heat-shock proteins, the Hsp70 molecular chaperone (DnaK), which is involved in protein stabilization. It has been shown in that an Hsp70 homologue called Hsc66, which is widespread in bacteria, functions as a chaperone . This paper reports the isolation of a W51D mutant (W51M22) by insertion of the mini-Tn-Hg transposon, which was unable to grow on ethanol and other short-chain alcohols as sole source of carbon. The transposon insertion in this mutant was shown to be located in the gene encoding Hsc66. The inability of mutant W51M22 to use ethanol was complemented by the operon. The authors characterized the transcriptional arrangement of , showing that it forms part of an operon with the upstream gene, and that it is also expressed from its own promoter. These results are compatible with the Hsc66 protein being a functional molecular chaperone involved in the stabilization, in the presence of ethanol, of some proteins required for bacterial growth on short-chain alcohols.

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2000-06-01
2019-12-08
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