1887

Abstract

The promoter of contains potential NtrC, NifA and IHF binding sites together with a −12/−24 σ-dependent promoter. This region has now been investigated by deletion mutagenesis for the effect of NtrC and NifA on the expression of a :: fusion. A 5’ end to the RNA was identified at position 641, 12 bp downstream from the −12/−24 promoter. Footprinting experiments showed that the G residues at positions −26 and −9 are hypermethylated, and that the region from −10 to +10 is partially melted under nitrogen-fixing conditions, confirming that this is the active promoter. In expression from the σ-dependent promoter is repressed by fixed nitrogen but not by oxygen and is probably activated by the NtrC protein. NifA protein is apparently not essential for expression but it can still bind the NifA upstream activating sequence.

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2000-06-01
2019-12-15
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