1887

Abstract

species are Gram-negative bacteria that cause a systemic infection in fish called vibriosis. The authors previously demonstrated that internalization and cytotoxicity are important virulence mechanisms in vibrio–fish epithelial cell interactions. Adherence is a prerequisite for successful internalization. In this study, the adherence capability of two invasive strains [ 811218–5W and G/Virus/5(3)] was compared with that of two non-invasive strains [ ATCC 33539 and S2/5/93(2)] using adherence assays in three different types of fish cells (epithelial papillosum of carp, EPC; grunt-fin tissue, GF; and fat-head minnow epithelial cells, FHM). For all four strains there was no significant difference (>005) in the adherence to the different cell lines. 811218–5W exhibited the highest adherence, followed by G/Virus/5(3) and S2/5/93(2); ATCC 33539 showed the lowest adherence. The super-adherence characteristic of 811218–5W on EPC cells was not affected by inhibitors, sugars, low temperature (4 °C) incubation, or non-biological surfaces such as glass coverslips. The galactose-linked adherence characteristic of G/Virus/5(3) to the EPC cells was partially inhibited by peptidase treatment of the fish cells, low-temperature incubation, and addition of sugars that contained galactose (such as lactose and -acetyl-D-galactosamine). synthesis of bacterial protein, viable bacteria and intact carbohydrate structure of vibrios were required for both super-adherence and galactose-linked adherence. These adherence characteristics were also found in ten other invasive vibrios, and galactose-linked adherence was found in nine invasive vibrios.

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2000-04-01
2019-10-23
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