1887

Abstract

Transcriptional analysis of five different regions of the 168 genome, comprising a total of 175 kb encoding newly identified genes, was carried out using the RT-PCR technique as part of the functional analysis of the whole genome of this bacterium. Amplification of mRNA fragments allowed the detection of both highly and poorly transcribed genes covering 81% of putative ORFs, and also the monitoring of variations in the expression level among genes differentially expressed during particular bacterial growth phases.

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/content/journal/micro/10.1099/00221287-146-4-823
2000-04-01
2019-12-14
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References

  1. Anagnostopoulos, C. & Spizizen, J. ( 1961; ). Requirements for transformation in Bacillus subtilis. J Bacteriol 81, 741-746.
    [Google Scholar]
  2. Beall, B., Driks, A., Losick, R. & Moran, C. P.Jr ( 1993; ). Cloning and characterization of a gene required for assembly of the Bacillus subtilis spore coat. J Bacteriol 175, 1705-1716.
    [Google Scholar]
  3. Chuang, S. E., Daniels, D. L. & Blatner, F. R. ( 1993; ). Global regulation of gene expression in Escherichia coli. J Bacteriol 175, 2026-2036.
    [Google Scholar]
  4. Harwood, C. R. & Cutting, S. M. (1990). Molecular Biological Methods for Bacillus. Chichester: Wiley.
  5. Kedzierski, W. & Porter, J. C. ( 1991; ). A novel non-enzymatic procedure for removing DNA template from RNA transcription mixtures. Biotechniques 10, 210-214.
    [Google Scholar]
  6. Kunst, F., Ogasawara, N., Moszer, I. & 148 other authors ( 1997; ). The complete genome sequence of the gram-positive bacterium Bacillus subtilis. Nature 390, 249–256.[CrossRef]
    [Google Scholar]
  7. Lockhart, D. J., Dong, H., Byrne, M. C. & 8 other authors ( 1996; ). Expression monitoring by hybridization to high-density oligonucleotide arrays. Nature Biotechnol 14, 1675–1680.[CrossRef]
    [Google Scholar]
  8. Mellado, R. P., Delius, H., Klein, B. & Murray, K. ( 1981; ). Transcription of sea urchin histone genes in Escherichia coli. Nucleic Acids Res 9, 3889-3906.[CrossRef]
    [Google Scholar]
  9. Pascal, M., Kunst, F., Lepesant, J. A. & Dedonder, R. ( 1971; ). Characterization of two sucrase activities in Bacillus subtilis Marburg. Biochimie 53, 1059-1066.[CrossRef]
    [Google Scholar]
  10. Pease, A. C., Solas, D., Sullivan, E. J., Cronin, M. T., Holmes, C. P. & Fodor, S. P. ( 1994; ). Light-directed oligonucleotide arrays for rapid DNA sequence analysis. Proc Natl Acad Sci USA 91, 5022-5026.[CrossRef]
    [Google Scholar]
  11. Plum, G. & Clark-Curtiss, J. E. ( 1994; ). Induction of Mycobacterium avium gene expression following phagocytosis by human macrophages. Infect Immun 62, 476-483.
    [Google Scholar]
  12. Sá-Nogueira, I. & Ramos, S. ( 1997; ). The Bacillus subtilis l-arabinose (ara) operon: nucleotide sequence, genetic organization and expression. Microbiology 143, 957-969.[CrossRef]
    [Google Scholar]
  13. de Sazieu, A., Certa, U., Warrington, J., Gray, C., Keck, W. & Mous, J. ( 1997; ). Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays. Nature Biotechnol 16, 45-48.
    [Google Scholar]
  14. Schaeffer, P., Millet, J. & Aubert, J.-P. ( 1965; ). Catabolic repression of bacterial sporulation. Proc Natl Acad Sci USA 54, 704-711.[CrossRef]
    [Google Scholar]
  15. Wodicka, L., Dong, H., Mittmann, M., Ho, M.-H. & Lockhart, D. ( 1997; ). Genome wide expression monitoring in Saccharomyces cerevisiae. Nature Biotechnol 15, 1359-1367.[CrossRef]
    [Google Scholar]
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