1887

Abstract

The gene encodes an 84 amino acid reading frame; in it is positioned between the and genes of the purine biosynthetic operon. Disruption of resulted in a purine-auxotrophic phenotype. When was expressed it was able to complement a mutation. Growth experiments and enzyme analysis of mutant strains revealed a defective phosphoribosylformylglycinamidine synthetase (FGAM synthetase). In the organisms in which FGAM synthetase has been studied a single polypeptide is responsible for activity. In some organisms two separate genes – in the and genes – encode polypeptides with similarity to the N-terminal and the C-terminal region, respectively, of the single-polypeptide FGAM synthetase. Thus, active FGAM synthetase in requires the gene product in addition to the and gene products. Open reading frames with sequence similarity to are found in other Gram-positive organisms, in a cyanobacterium and in methanogenic archaea. The designation is proposed for this novel function in purine biosynthesis in .

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2000-04-01
2020-04-06
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