1887

Abstract

P27 is an antigenic membrane lipoprotein synthesized by members of the complex. Northern blotting and RT-PCR experiments indicated that the genes encoding P27 and a putative antibiotic transporter (P55) form an operon. A promoter region was identified and characterized by deletion analysis in . Two transcription initiation points were mapped in BCG by primer extension analysis to 76 bp and 87 bp upstream of the ATG initiation codon. Putative −10 and −35 promoter consensus sequences associated with these showed 66% similarity to previously identified mycobacterial promoters. These results suggest that the / operon is transcribed from two promoters in BCG.

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2000-04-01
2020-08-05
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