1887

Abstract

Glucose and mannose are transported in streptococci by the mannose-PTS (phosphoenolpyruvate:mannose phosphotransferase system), which consists of a cytoplasmic IIAB protein, called IIAB, and an uncharacterized membrane permease. This paper reports the characterization of the operon encoding the specific components of the mannose-PTS of . The operon was composed of four genes, , , and . These genes were transcribed from a canonical promoter () into a 36 kb polycistronic mRNA that contained a 5′-UTR (untranslated region). The predicted gene product encoded a 355 kDa protein and contained the amino acid sequences of the IIA and IIB phosphorylation sites already determined from purified . Expression of in generated a 35 kDa protein that reacted with antibodies. The predicted ManM protein had an estimated size of 272 kDa. ManM had similarity with IIC domains of the mannose-EII family, but did not possess the signature proposed for mannose-IIC proteins from Gram-negative bacteria. From multiple alignment analyses of sequences available in current databases, the following modified IIC signature is proposed: GXG[DNH]XG[LIVM]XG[STL][LT][EQ]. The deduced product of was a hydrophobic protein with a predicted molecular mass of 334 kDa. The ManN protein contained an amino acid sequence similar to the signature sequence of the IID domains of the mannose-EII family. encoded a 137 kDa protein. This gene was also transcribed as a monocistronic mRNA from a promoter located in the intergenic region. A search of current databases revealed the presence of , ManM, ManN and ManO orthologues in , , and . This work has elucidated the molecular structure of the mannose PTS in streptococci and enterococci, and demonstrated the presence of a putative regulatory protein (ManO) within the operon.

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2000-03-01
2020-01-26
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