1887

Abstract

A 5·7 kbp I fragment containing the polyvinyl alcohol (PVA) dehydrogenase gene and its 19 kbp 5′-flanking region was cloned from the PVA-degrading bacterium sp. VM15C. The gene, encoding oxidized PVA hydrolase, was found in the region upstream of . Sequence data and expression studies indicated that and constitute an operon in the order . The gene encoded a protein of 379 amino acid residues (40610 Da), and a lipoprotein signal sequence and the lipase consensus sequence, Gly-X-Ser-X-Gly, characteristic of the active-site serine region in serine hydrolases, were detected in the deduced amino acid sequence. The product with the product constituted an enzyme system for the cleavage of PVA molecules. The product introduced β-diketone groups into the PVA molecule, and the product hydrolysed these β-diketone groups in oxidized PVA. The product also hydrolysed 4,6-nonanedione at a low rate, but not acetylacetone or 5-nonanone. It was completely inhibited by PMSF and was concluded to be a serine hydrolase. There were no proteins showing high similarity to the product in the databases, but minor similarity to a number of serine hydrolases including polyhydroxyalkanoate depolymerases was apparent.

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/content/journal/micro/10.1099/00221287-146-3-649
2000-03-01
2019-10-21
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