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Volume 146, Issue 3

Overproduction of the secretin OutD suppresses the secretion defect of an mutant Free

Abstract

OutB is a component of the Out secretion machinery. Homologues of OutB have been described in two other bacteria, and , but their requirement in the secretion process seems to be different. Study of OutB topology with the BlaM topology probe suggests that it is an inner-membrane protein with a large periplasmic domain. However, fractionation experiments indicate that it could be associated with the outer membrane through its C-terminal part. The secretion deficiency of an mutant can be reversed by the addition of an inducer of the regulon. It was shown that this effect results from the increased expression of the secretin OutD and that secretion can be restored in an mutant by introducing the gene on a plasmid. Several experiments suggest an interaction between OutB and OutD. In , the presence of OutD stabilizes OutB. OutD expressed in can be protected from proteolytic degradation by the coexpression of OutB. This effect does not require the N-terminal, transmembrane segment of . OutB can be cross-linked with OutD by formaldehyde. These results indicate that OutB could act with OutD in the functioning of the Out secretion machinery.

  • Received:
  • Accepted:
  • Published Online:
Keyword(s): CMC, carboxymethylcellulose , general secretory pathway , GSP, general secretory pathway , PGA, polygalacturonate , protein secretion , protein–protein interaction and secretin
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2000-03-01
2024-04-25
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Overproduction of the secretin OutD suppresses the secretion defect of an Erwinia chrysanthemi outB mutant
Microbiology 146, 639 (2000); https://doi.org/10.1099/00221287-146-3-639
/content/journal/micro/10.1099/00221287-146-3-639
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