RT Journal Article SR Electronic(1) A1 Seegers, Jos F. M. L. A1 van Sinderen, Douwe A1 Fitzgerald, Gerald F. YR 2000 T1 Molecular characterization of the lactococcal plasmid pCIS3: natural stacking of specificity subunits of a type I restriction/modification system in a single lactococcal strainThe GenBank accession numbers for the sequences reported in this paper are AF153410–AF153414. JF Microbiology, VO 146 IS 2 SP 435 OP 443 DO https://doi.org/10.1099/00221287-146-2-435 PB Microbiology Society, SN 1465-2080, AB A 6·1 kb plasmid from the Lactococcus lactis subsp. cremoris strain UC509.9, named pCIS3, was found to mediate a restriction/modification (R/M) phenotype. Nucleotide sequence analysis of pCIS3 revealed the presence of an hsdS gene, typical of type I R/M systems. The presence of this plasmid resulted in a 104-fold reduction in the efficiency of plating (e.o.p.) of unmodified phage. In addition to the hsdS gene of pCIS3, two more hsdS genes were identified in strain UC509.9, one located on the chromosome downstream of a gene highly homologous to hsdM genes and a third on the smallest (4 kb) plasmid, named pCIS1. The replication region of pCIS3 was highly similar to that of a large family of lactococcal theta replicons. In addition, pCIS3 was found to encode a member of the CorA family of magnesium transporters., UL https://www.microbiologyresearch.org/content/journal/micro/10.1099/00221287-146-2-435