1887

Abstract

A 6·1 kb plasmid from the subsp. strain UC509.9, named pCIS3, was found to mediate a restriction/modification (R/M) phenotype. Nucleotide sequence analysis of pCIS3 revealed the presence of an gene, typical of type I R/M systems. The presence of this plasmid resulted in a 10-fold reduction in the efficiency of plating (e.o.p.) of unmodified phage. In addition to the gene of pCIS3, two more genes were identified in strain UC509.9, one located on the chromosome downstream of a gene highly homologous to genes and a third on the smallest (4 kb) plasmid, named pCIS1. The replication region of pCIS3 was highly similar to that of a large family of lactococcal theta replicons. In addition, pCIS3 was found to encode a member of the CorA family of magnesium transporters.

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2000-02-01
2024-04-18
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