1887

Abstract

A 6·1 kb plasmid from the subsp. strain UC509.9, named pCIS3, was found to mediate a restriction/modification (R/M) phenotype. Nucleotide sequence analysis of pCIS3 revealed the presence of an gene, typical of type I R/M systems. The presence of this plasmid resulted in a 10-fold reduction in the efficiency of plating (e.o.p.) of unmodified phage. In addition to the gene of pCIS3, two more genes were identified in strain UC509.9, one located on the chromosome downstream of a gene highly homologous to genes and a third on the smallest (4 kb) plasmid, named pCIS1. The replication region of pCIS3 was highly similar to that of a large family of lactococcal theta replicons. In addition, pCIS3 was found to encode a member of the CorA family of magnesium transporters.

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2000-02-01
2020-01-28
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References

  1. Allison G. E., Klaenhammer T. R.. 1998; Phage resistance mechanisms in lactic acid bacteria. Int Dairy J8:207–226[CrossRef]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search program. Nucleic Acid Res25:3389–3402[CrossRef]
    [Google Scholar]
  3. Arendt E. K., Daly C., Fitzgerald G. F., van de Guchte M.. 1994; Molecular characterization of lactococcal bacteriophage Tuc2009 and identification and analysis of genes encoding lysin, a putative holin, and two structural proteins. Appl Environ Microbiol60:1875–1883
    [Google Scholar]
  4. Bidnenko E., Ehrlich D., Chopin M. C.. 1995; Phage operon involved in sensitivity to the Lactococcus lactis abortive infection mechanism AbiD1. J Bacteriol177:3824–3829
    [Google Scholar]
  5. Bult C. J., White O., Olsen G. J..20 other authors 1996; Complete genome sequence of the methanogenic archaeon,. Methanococcus jannaschii. Science273:1058–1073
    [Google Scholar]
  6. Chopin A., Chopin M. C., Moillo-Bat A., Langella P.. 1984; Two plasmid-determined restriction and modification systems in Streptococcus lactis. Plasmid11:260–263[CrossRef]
    [Google Scholar]
  7. Costello V. A.. 1988; Characterization of bacteriophage–host interactions in Streptococcus cremoris UC503 and related lactic streptococci PhD thesis National University of Ireland, University College Cork; Ireland:
    [Google Scholar]
  8. Dagert M., Ehrlich S. D.. 1979; Prolonged incubation in calcium chloride improves competence of Escherichia coli cells. Gene6:23–28[CrossRef]
    [Google Scholar]
  9. Davis R., van der Lelie D., Mereenier A., Daly C., Fitzgerald G. F.. 1993; ScrFI restriction-modification system of Lactococcus lactis subsp. cremoris UC503: cloning and characterization of two ScrFI methylase genes. Appl Environ Microbiol59:777–785
    [Google Scholar]
  10. Dickely F., Nilsson D., Hansen E. B., Johansen E.. 1995; Isolation of Lactococcus lactis nonsense suppressors and construction of a food-grade cloning vector. Mol Microbiol15:839–847[CrossRef]
    [Google Scholar]
  11. Foley S., Bron S., Venema G., Daly C., Fitzgerald G. F.. 1996; Molecular analysis of the replication origin of the Lactococcus lactis plasmid pCJ305. Plasmid36:125–141[CrossRef]
    [Google Scholar]
  12. Forde A., Daly C., Fitzgerald G. F.. 1999; Identification of four phage resistance plasmids from Lactococcus lactis subsp. cremoris HO2. Appl Environ Microbiol65:1540–1547
    [Google Scholar]
  13. Fuller-Pace F. V., Murray N. E.. 1986; Two DNA recognition domains of the specificity polypeptides of a family of type 1 restriction enzymes. Proc Natl Acad Sci USA83:9368–9372[CrossRef]
    [Google Scholar]
  14. Fuller-Pace F. V., Bullas L. R., Delius H., Murray N. E.. 1984; Genetic recombination can generate altered restriction specificity. Proc Natl Acad Sci USA81:6095–6099[CrossRef]
    [Google Scholar]
  15. Garvey P., van Sinderen D., Twomey D. P., Hill C., Fitzgerald G. F.. 1995; Molecular genetics of bacteriophages and natural phage defense systems in the genus Lactococcus. Int Dairy J5:905–947[CrossRef]
    [Google Scholar]
  16. Garvey P., Hill C., Fitzgerald G. F.. 1996; The lactococcal plasmid pNP40 encodes a third bacteriophage resistance mechanism, one which affects phage DNA penetration. Appl Environ Microbiol62:676–679
    [Google Scholar]
  17. Gravesen A., von Wright A., Josephsen J., Vogensen F. K.. 1997; Replication regions of two pairs of incompatible lactococcal theta-replicating plasmids. Plasmid38:115–127[CrossRef]
    [Google Scholar]
  18. Hayes F., Vos P., Fitzgerald G. F., de Vos W. M., Daly C.. 1991; Molecular organization of the minimal replicon of novel, narrow-host-range, lactococcal plasmid pCI305. Plasmid25:16–26[CrossRef]
    [Google Scholar]
  19. von Heijne G.. 1992; Membrane protein structure prediction, hydrophobicity analysis and the positive-inside rule. J Mol Biol225:487–494[CrossRef]
    [Google Scholar]
  20. Kehres D. G., Lawyer C. H., Maguire M. E.. 1998; The CorA magnesium transporter gene family. Microb Comp Genomics3:151–169[CrossRef]
    [Google Scholar]
  21. Lillehaug D.. 1997; An improved plaque assay for poor plaque-producing temperate lactococcal bacteriophages. J Appl Microbiol83:85–90[CrossRef]
    [Google Scholar]
  22. McKay L. L.. 1983; Functional properties of plasmids in lactic streptococci. Antonie Leeuwenhoek49:259–274[CrossRef]
    [Google Scholar]
  23. O’Sullivan D. J., Zagula K., Klaenhammer T. R.. 1995; In vivo restriction by LlaI is encoded by three genes, arranged in an operon with llaIM, on the conjugative Lactococcus plasmid pTR2030. J Bacteriol177:134–143
    [Google Scholar]
  24. O’Sullivan T., van Sinderen D., Fitzgerald G.. 1999; Structural and functional analysis of pCI65st, a 6·5 kb plasmid from Streptococcus thermophilus NDI-6. Microbiology145:127–134[CrossRef]
    [Google Scholar]
  25. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  26. Schouler C., Clier F., Lerayer A. L., Ehrlich S. D., Chopin M. C.. 1998a; A type IC restriction-modification system in Lactococcus lactis. J Bacteriol180:407–411
    [Google Scholar]
  27. Schouler C., Gautier M., Ehrlich S. D., Chopin M. C.. 1998b; Combinational variation of restriction modification specificities in Lactococcus lactis. Mol Microbiol28:169–178
    [Google Scholar]
  28. Seegers J. F., Bron S., Franke C. M., Venema G., Kiewiet R.. 1994; The majority of lactococcal plasmids carry a highly related replicon. Microbiology140:1291–1300[CrossRef]
    [Google Scholar]
  29. Smith R. L., Maguire M. E.. 1998; Microbial magnesium transport: unusual transporters searching for identity. Mol Microbiol28:217–226[CrossRef]
    [Google Scholar]
  30. Su P., Im H., Hsieh H., Kang A. S., Dunn N. W.. 1999; LlaFI, a type III restriction and modification system in Lactococcus lactis. Appl Environ Microbiol65:686–693
    [Google Scholar]
  31. Van der Vossen J. M., van der Lelie D., Venema G.. 1987; Isolation and characterization of Streptococcus cremoris Wg2-specific promoters. Appl Environ Microbiol53:2452–2457
    [Google Scholar]
  32. Wells J. M., Wilson P. W., Le Page R. W.. 1993; Improved cloning vectors and transformation procedure for Lactococcus lactis. J Appl Bacteriol74:629–636[CrossRef]
    [Google Scholar]
  33. Yuan R.. 1981; Structure and mechanism of multifunctional restriction endonucleases. Annu Rev Biochem80:285–315
    [Google Scholar]
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