1887

Abstract

A novel valanimycin-resistance determinant () was isolated from a cosmid containing DNA that leads to valanimycin production in . Expression of the gene in both and provided valanimycin resistance. The nucleotide sequence of consists of 1206 bp and the deduced amino acid sequence encodes a polypeptide with 12 putative transmembrane-spanning segments and a calculated pI of 101. VlmF shows significant similarities to other known or putative transmembrane efflux proteins that confer antibiotic resistance, but it appears to be specific for valanimycin. The sequence similarities suggest that VlmF is a member of the DHA12 family within the major facilitator superfamily of transport proteins and that it is probably involved in active valanimycin efflux energized by a proton-dependent electrochemical gradient.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-2-345
2000-02-01
2020-01-28
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/2/1460345a.html?itemId=/content/journal/micro/10.1099/00221287-146-2-345&mimeType=html&fmt=ahah

References

  1. Baltz R. H.. 1980; Genetic recombination by protoplast fusion in Streptomyces. Dev Ind Microbiol21:43–54
    [Google Scholar]
  2. Bibb M. J., Cohen S. N.. 1982; Gene expression in Streptomyces: construction and application of promoter-probe plasmid vectors in Streptomyces lividans. Mol Gen Genet187:265–277[CrossRef]
    [Google Scholar]
  3. Bierman M., Logan R., O’Brien K., Seno E. T., Rao R. N., Schoner B. E.. 1992; Plasmid cloning vectors for the conjugal transfer of DNA from Escherichia coli to Streptomyces spp. Gene116:43–49[CrossRef]
    [Google Scholar]
  4. Chiu M. L., Folcher M., Griffin P., Holt T., Klatt T., Thompson C. J.. 1996; Characterization of the covalent binding of thiostrepton to a thiostrepton-induced protein from Streptomyces lividans. Biochemistry35:2332–2341[CrossRef]
    [Google Scholar]
  5. Cundliffe E.. 1989; How antibiotic-producing organisms avoid suicide. Annu Rev Microbiol43:207–233[CrossRef]
    [Google Scholar]
  6. Fujiu M., Sawairi S., Shimada H., Takaya H., Aoki Y., Okuda T., Yokose K.. 1994; Azoxybacilin, a novel antifungal agent produced by Bacillus cereus NR2991. J Antibiot47:833–835[CrossRef]
    [Google Scholar]
  7. Henderson P. J. F., Maiden M. C. J.. 1990; Homologous sugar transport proteins in Escherichia coli and their relatives in both prokaryotes and eukaryotes. Philos Trans R Soc Lond Ser B326:391–410[CrossRef]
    [Google Scholar]
  8. Hopwood D. A., Bibb M. J., Chater K. F..7 other authors 1985; Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  9. Imamura N., Kuga H., Otoguro K., Tanaka H., Omura S.. 1989; Structures of jietacins: unique α,β-unsaturated azoxy antibiotics. J Antibiot42:156–158[CrossRef]
    [Google Scholar]
  10. Korsch B., Riggs N. V.. 1964; Proton magnetic resonance spectra of aliphatic azoxy compounds. The structure of cycasin. Tetrahedron Lett523:525
    [Google Scholar]
  11. Kyte J., Doolittle R. F.. 1982; A simple method for displaying the hydropathic character of a protein. J Mol Biol157:105–132[CrossRef]
    [Google Scholar]
  12. Langley B. W., Lythgoe B., Riggs N. V.. 1951; Macrozamin pt II. The presence of linked nitrogen atoms in the aglycone part. Chem Ind (Lond)75:
    [Google Scholar]
  13. Lythgoe B., Riggs N. V.. 1949; Macrozamin pt I. The identity of the carbohydrate component. J Chem Soc2716:2718
    [Google Scholar]
  14. Mazodier P., Petter R., Thompson C.. 1989; Intergeneric conjugation between Escherichia coli and Streptomyces species. J Bacteriol171:3583–3585
    [Google Scholar]
  15. Pao S. S., Paulsen I. A., Saier M. H. Jr. 1998; Major facilitator superfamily. Microbiol Mol Biol Rev62:1–34
    [Google Scholar]
  16. Parry R. J., Li W.. 1997; An NADPH:FAD oxidoreductase from the valanimycin producer Streptomyces viridifaciens: cloning, analysis, and overexpression. J Biol Chem272:23303–23311[CrossRef]
    [Google Scholar]
  17. Parry R. J., Li Y., Lii F.-W.. 1992; Biosynthesis of azoxy compounds. Investigations of valanimycin biosynthesis. J Am Chem Soc114:10062–10064[CrossRef]
    [Google Scholar]
  18. Parry R. J., Li W., Cooper H. N.. 1997; Cloning, analysis, and overexpression of the gene encoding isobutylamine N-hydroxylase from the valanimycin producer, Streptomyces viridifaciens. J Bacteriol179:409–416
    [Google Scholar]
  19. Paulsen I. T., Brown M. H., Skurray R. A.. 1996; Proton-dependent multidrug efflux systems. Microbiol Rev60:575–608
    [Google Scholar]
  20. Riggs N. V.. 1956; Glucosyloxyazoxymethane, a constituent of the seeds of Cycas circinalis. Chem Ind (Lond)926:
    [Google Scholar]
  21. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  22. Simon R., Priefer U., Puhler A.. 1983; A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in gram negative bacteria. Bio/Technology1:784–791[CrossRef]
    [Google Scholar]
  23. Stevens C. L., Gillis B. T., French J. C., Haskell T. H.. 1956; The structure of elaiomycin, a tuberculostatic antibiotic. J Am Chem Soc78:3229–3230[CrossRef]
    [Google Scholar]
  24. Stevens C. L., Gillis B. T., French J. C., Haskell T. H.. 1958; Elaiomycin. An aliphatic α,β-unsaturated azoxy compound. J Am Chem Soc80:6088–6092[CrossRef]
    [Google Scholar]
  25. Stevens C. L., Gillis B. T., Haskell T. H.. 1959; Elaiomycin. II. Determination of the d-threo configuration. J Am Chem Soc81:1435–1437[CrossRef]
    [Google Scholar]
  26. Takahashi Y., Nakayama M., Watanabe I., Deushi T., Ishiwata H., Shiratsuchi M., Otani G.. 1989; Novel antifungal antibiotics maniwamycins A and B. II. Structure determination. J Antibiot42:1541–1546[CrossRef]
    [Google Scholar]
  27. Tang L., Grimm A., Zhang Y. X., Hutchinson C. R.. 1996; Purification and characterization of the DNA-binding protein Dnrl, a transcriptional factor of daunorubicin biosynthesis in Streptomyces peucetius. Mol Microbiol22:801–813[CrossRef]
    [Google Scholar]
  28. Tercero J., Lacalle R., Jimenez A.. 1993; The pur8 gene from the pur cluster of Streptomyces alboniger encodes a highly hydrophobic polypeptide which confers resistance to puromycin. Eur J Biochem218:963–971[CrossRef]
    [Google Scholar]
  29. Wright F., Bibb M.. 1992; Codon usage in the G+C-rich Streptomyces genome. Gene113:55–65[CrossRef]
    [Google Scholar]
  30. Yamaguchi A., Ono N., Akasaka T., Noumi T., Sawai T.. 1990; Metal-tetracycline/H+ antiporter of Escherichia coli encoded by a transposon, Tn10. J Biol Chem265:15525–15530
    [Google Scholar]
  31. Yamaguchi A., Someya Y., Sawai T.. 1992; Metal-tetracycline/H+ antiporter of Escherichia coli encoded by transposon Tn10. J Biol Chem267:19155–19162
    [Google Scholar]
  32. Yamato M., Iinuma H., Naganawa H., Yamagishi Y., Hamada M., Masuda T., Umezawa H.. 1986; Isolation and properties of valanimycin, a new azoxy antibiotic. J Antibiot39:184–191[CrossRef]
    [Google Scholar]
  33. Yamato M., Umezawa H., Sakata N., Moriya Y., Hori M.. 1987; Valanimycin acts on DNA in bacterial cells. J Antibiot40:558–560[CrossRef]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-2-345
Loading
/content/journal/micro/10.1099/00221287-146-2-345
Loading

Data & Media loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error