1887

Abstract

The sporulation gene of was shown [Davis, N. K. & Chater, K. F. (1992) . 232, 351–358] to encode a small, cysteine-rich putative transcription factor unlike any that had been described previously. The large database of DNA sequences of mycobacteria (like , members of the Actinomycetales) has revealed a family of genes encoding proteins related to WhiB. contains at least six such genes (omologues in ycobacteria: ) and a likely seventh, . Using conserved features of Whm proteins, a PCR-based approach led to the discovery that A3(2) contains several similar genes. Cloning and sequencing of these -ike () genes revealed likely orthologues of four of the genes of . In all, contains at least five genes in addition to itself. All five were shown by RT-PCR to be transcribed. A Southern blotting survey using each gene as a probe showed that nearly all of a series of representatives of ten actinomycete genera (including morphologically simple organisms) contain close homologues of several genes, suggesting that the ancient progenitor of all these organisms already contained a family of such genes, which have not been found in any other organisms.

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2000-02-01
2020-01-20
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References

  1. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402[CrossRef]
    [Google Scholar]
  2. Chater K. F.. 1972; A morphological and genetic mapping study of white colony mutants of Streptomyces coelicolor. J Gen Microbiol72:9–28[CrossRef]
    [Google Scholar]
  3. Chater K. F.. 1993; Genetics of differentiation in Streptomyces. Annu Rev Microbiol47:685–713[CrossRef]
    [Google Scholar]
  4. Chater K. F.. 1998; Taking a genetic scalpel to the Streptomyces colony. Microbiology144:1465–1478[CrossRef]
    [Google Scholar]
  5. Chater K. F., Losick R.. 1997; The mycelial life-style of Streptomyces coelicolor A3(2) and its relatives. In Bacteria as Multicellular Organisms pp.149–182Edited by Shapiro J. H., Dworkin M.. New York: Oxford University Press;
    [Google Scholar]
  6. Chatterjee B. R.. 1976; Carrier state in leprosy. Lepr India48:643–644
    [Google Scholar]
  7. Cole S. T., Brosch R., Parkhill J..39 other authors 1998; Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence. Nature393:537–544[CrossRef]
    [Google Scholar]
  8. Davis N. K., Chater K. F.. 1992; The Streptomyces coelicolor whiB gene encodes a small transcription factor-like protein dispensable for growth but essential for sporulation. Mol Gen Genet232:351–358
    [Google Scholar]
  9. Daza A., Gil J. A., Vigal T., Martin J. F.. 1990; Cloning and characterization of a gene of Streptomyces griseus that increases production of extracellular enzymes in several species of Streptomyces. Mol Gen Genet222:384–392[CrossRef]
    [Google Scholar]
  10. Eiglmeier K., Honoré N., Woods S. A., Caudron B., Cole S. T.. 1993; Use of an ordered cosmid library to deduce the genomic organization of Mycobacterium leprae. Mol Microbiol7:197–206[CrossRef]
    [Google Scholar]
  11. Embley T. M., Stackebrandt E.. 1994; The molecular phylogeny and systematics of the actinomycetes. Annu Rev Microbiol48:257–289[CrossRef]
    [Google Scholar]
  12. Felsenstein J.. 1988; Phylogenies from molecular sequences: inference and reliability. Annu Rev Genet22:521–565[CrossRef]
    [Google Scholar]
  13. Ford M. E., Stenstrom C., Hendrix R. W., Hatfull G. F.. 1998; Mycobacteriophage TM4: genome structure and gene expression. Tuber Lung Dis79:63–73[CrossRef]
    [Google Scholar]
  14. Fsihi H., De Rossi E., Salazar L..7 other authors 1996; Gene arrangement and organization in a ∼76 kb fragment encompassing the oriC region of the chromosome of Mycobacterium leprae. Microbiology142:3147–3161[CrossRef]
    [Google Scholar]
  15. Gaudu P., Moon N., Weiss B.. 1997; Regulation of the soxRS oxidative stress region: reversible oxidation of the Fe–S centres of SoxR. J Biol Chem272:5082–5086[CrossRef]
    [Google Scholar]
  16. Hanahan D.. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol166:557–580[CrossRef]
    [Google Scholar]
  17. Holland P. W.. 1999; The effect of gene duplication on homology. Novartis Found Symp222:226–236
    [Google Scholar]
  18. Honor é N., Bergh S., Chanteau S..7 other authors 1993; Nucleotide sequence of the first cosmid from the Mycobacterium leprae genome project: structure and function of the Rif–Str regions. Mol Microbiol7:207–214[CrossRef]
    [Google Scholar]
  19. Hopwood D. A., Bibb M. J., Chater K. F..7 other authors 1985; Genetic Manipulation of Streptomyces: a Laboratory Manual Norwich: John Innes Foundation;
    [Google Scholar]
  20. Hutter B., Dick T.. 1999; Molecular genetic characterisation of whiB3, a mycobacterial homologue of a Streptomyces sporulation factor. Res Microbiol150:295–301[CrossRef]
    [Google Scholar]
  21. Janssen G. R., Bibb M. J.. 1993; Derivatives of pUC18 that have BglII sites flanking a modified multiple cloning site and that retain the ability to identify recombinant clones by visual screening of Escherichia coli colonies. Gene124:133–134[CrossRef]
    [Google Scholar]
  22. Julien B., Pountney D., Christie G. E., Calendar R.. 1998; Mutational analysis of a satellite phage activator. Gene223:129–134[CrossRef]
    [Google Scholar]
  23. Kelemen G. H., Brown G. L., Kormanec J., Potuckova L., Chater K. F., Buttner M. J.. 1996; The positions of the sigma factor genes, whiG and sigF, in the hierarchy controlling the development of spore chains in the aerial hyphae of Streptomyces coelicolor A3(2). Mol Microbiol21:593–603[CrossRef]
    [Google Scholar]
  24. Kormanec J., Sevcikova B., Sprusansky O., Benada O., Kofronova O., Novakova R., Rezuchova B., Potuckova L., Homerova D.. 1998; The Streptomyces aureofaciens homologue of the whiB gene is essential for sporulation; its expression correlates with the developmental stage. Folia Microbiol43:605–612[CrossRef]
    [Google Scholar]
  25. Lim A., Eleuterio M., Hutter B., Murugasu-Oei B., Dick T.. 1999; Oxygen depletion-induced dormancy in Mycobacterium bovis BCG. J Bacteriol181:2252–2256
    [Google Scholar]
  26. Mulder N. J., Zappe H., Steyn L. M.. 1999; Characterization of a Mycobacterium tuberculosis homologue of the Streptomyces coelicolor whiB gene. Tuber Lung Dis78:299–308
    [Google Scholar]
  27. Newton G. L., Arnold K., Price M. S..7 other authors 1996; Distribution of thiols in microorganisms: mycothiol is a major thiol in most actinomycetes. J Bacteriol178:1990–1995
    [Google Scholar]
  28. Nyström T.. 1999; Starvation, cessation of growth and bacterial aging. Curr Opin Microbiol2:214–219[CrossRef]
    [Google Scholar]
  29. Ochman H., Wilson A. C.. 1987; Evolutionary history of enteric bacteria. In Escherichia coli and Salmonella typhimurium: Cellular and Molecular Biology pp.1649–1654Edited by Neidhardt F. C..others Washington, DC: American Society for Microbiology;
    [Google Scholar]
  30. O’Halloran T. V.. 1993; Transition metals in control of gene expression. Science261:715–725[CrossRef]
    [Google Scholar]
  31. Parrish N., Dick J., Bishai W. R.. 1998; Mechanisms of latency in Mycobacterium tuberculosis. Trends Microbiol6:107–112[CrossRef]
    [Google Scholar]
  32. Redenbach M., Kieser H. M., Denapaite D., Eichner A., Cullum J., Kinashi H., Hopwood D. A.. 1996; A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome. Mol Microbiol21:77–96[CrossRef]
    [Google Scholar]
  33. Roller C., Ludwig W., Schleifer K. H.. 1992; Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes. J Gen Microbiol138:1167–1175[CrossRef]
    [Google Scholar]
  34. Sambrook J., Fritsch E. F., Maniatis T.. 1989; Molecular Cloning: a Laboratory Manual, 2nd edn. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory;
    [Google Scholar]
  35. Schjerling P., Holmberg S.. 1996; Comparative amino acid sequence analysis of the C6 zinc cluster family of transcription regulators. Nucleic Acids Res24:4599–4607[CrossRef]
    [Google Scholar]
  36. Seibert V., Kourbatova E. M., Golovleva L. A., Schlomann M.. 1998; Characterization of the maleylacetate reductase MacA of Rhodococcus opacus 1CP and evidence for the presence of an isofunctional enzyme. J Bacteriol180:3503–3508
    [Google Scholar]
  37. Smith D. R., Richterich P., Rubenfield M..22 other authors 1997; Multiplex sequencing of 1·5 Mb of the Mycobacterium leprae genome. Genome Res7:802–819
    [Google Scholar]
  38. Soliveri J., Granozzi C., Plaskitt K. A., Chater K. F.. 1993; Functional and evolutionary implications of a survey of various actinomycetes for homologues of two Streptomyces coelicolor sporulation genes. J Gen Microbiol139:2569–2578[CrossRef]
    [Google Scholar]
  39. Suzuki M.. 1993; Common features in DNA recognition helices of eukaryotic transcription factors. EMBO J12:3221–3226
    [Google Scholar]
  40. Zheng M., Åslund F., Storz G.. 1998; Activation of the OxyR transcription factor by reversible disulfide bond formation. Science279:1718–1721[CrossRef]
    [Google Scholar]
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