1887

Abstract

The sporulation gene of was shown [Davis, N. K. & Chater, K. F. (1992) . 232, 351–358] to encode a small, cysteine-rich putative transcription factor unlike any that had been described previously. The large database of DNA sequences of mycobacteria (like , members of the Actinomycetales) has revealed a family of genes encoding proteins related to WhiB. contains at least six such genes (omologues in ycobacteria: ) and a likely seventh, . Using conserved features of Whm proteins, a PCR-based approach led to the discovery that A3(2) contains several similar genes. Cloning and sequencing of these -ike () genes revealed likely orthologues of four of the genes of . In all, contains at least five genes in addition to itself. All five were shown by RT-PCR to be transcribed. A Southern blotting survey using each gene as a probe showed that nearly all of a series of representatives of ten actinomycete genera (including morphologically simple organisms) contain close homologues of several genes, suggesting that the ancient progenitor of all these organisms already contained a family of such genes, which have not been found in any other organisms.

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2000-02-01
2019-12-06
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