1887

Abstract

The insertion sequence IS is linked to the regions of both O1 and O139, and its location was suggestive of a role in generating new combinations of genes. This provoked an examination of the distribution and localization of IS in . IS was widely distributed in a number of serogroups. In particular, when cosmid clones of O1 were screened with IS and subsequently subcloned and sequenced, it was found that like genes were linked to this region. Furthermore, when the previously identified genes and from O1, now known to be involved in LPS biosynthesis, were used as probes, it was discovered that they too are present on the same large RI fragment as IS. This clearly indicated that IS was linked to the region of O1. Further analysis of the location of IS in other serotypes indicated that O2 also has IS associated with -like genes. In O2 there is more than one copy of IS, suggesting that this element is a site for recombination, gene duplication or that it may be capable of transposition. Following this latter premise, IS elements from a variety of strains have been cloned and sequenced. Only those strains with multiple copies of IS produce a full-length putative transposase, as shown by protein overexpression, further strengthening the argument that the element is transposing within these strains.

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2000-02-01
2019-09-22
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