1887

Abstract

Linear plasmids have previously been identified by the authors in mycobacteria, the telomeres of which have terminal inverted repeats and covalently attached proteins. In this study, the replication of these unusual molecules was investigated by studying a 25 kb linear plasmid from the slow-growing species called pCLP. An internal region of pCLP responsible for replication in was identified. The nucleotide sequence of the minimum replication region of pCLP, which was 28 kb long, contained a putative replication gene, , and a putative origin of replication consisting of an 18 bp direct repeat and an AT-rich region. A short section of the pCLP replication region was also found to have sequence identity with the replication regions of mycobacterial circular plasmids, suggesting that these linear and circular plasmids are related. It was found that pCLP replicated in BCG and was compatible in with pAL5000- and pJAZ38-derived plasmids from , which belong to two different compatibility groups. Thus, this new –mycobacteria shuttle vector may be used in both slow- and fast-growing mycobacteria and in co-transformation experiments with other mycobacterial vectors.

Loading

Article metrics loading...

/content/journal/micro/10.1099/00221287-146-2-305
2000-02-01
2020-04-02
Loading full text...

Full text loading...

/deliver/fulltext/micro/146/2/1460305a.html?itemId=/content/journal/micro/10.1099/00221287-146-2-305&mimeType=html&fmt=ahah

References

  1. Ainsa J. A., Perez E., Pelicic V., Berthet F. X., Gicquel B., Martin C.. 1997; Aminoglycoside 2′-N-acetyltransferase genes are universally present in mycobacteria: characterization of the aac(2′)-lc gene from Mycobacterium tuberculosis and the aac(2′)-ld gene from Mycobacterium smegmatis. Mol Microbiol24:431–441[CrossRef]
    [Google Scholar]
  2. Altschul S. F., Madden T. L., Schaffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. 1997; Gapped blast and psi-blast: a new generation of protein database search programs. Nucleic Acids Res25:3389–3402[CrossRef]
    [Google Scholar]
  3. Beggs M. L., Crawford J. T., Eisenach K. D.. 1995; Isolation and sequencing of the replication region of Mycobacterium avium plasmid pLR7. J Bacteriol17:4836–4840
    [Google Scholar]
  4. Brennan R. G., Matthews B. W.. 1989; The helix–turn–helix DNA binding motif. J Biol Chem264:1903–1906
    [Google Scholar]
  5. Chang P. C., Cohen S. N.. 1994; Bidirectional replication from an internal origin in a linear Streptomyces plasmid. Science265:952–954[CrossRef]
    [Google Scholar]
  6. Chang P. C., Kim E. S., Cohen S. N.. 1996; Streptomyces linear plasmids that contain a phage-like, centrally located, replication origin. Mol Microbiol22:789–800[CrossRef]
    [Google Scholar]
  7. Crespi M., Messens E., Caplan A. B., van Montagu M., Desomer J.. 1992; Fasciation induction by the phytopathogen Rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. EMBO J11:795–804
    [Google Scholar]
  8. Dabrock B., Kebeler M., Averhoff B., Gottschalk G.. 1994; Identification and characterization of a transmissible linear plasmid from Rhodococcus erythropolis BD2 that encodes isopropylbenzene and trichloroethene catabolism. Appl Environ Microbiol60:853–860
    [Google Scholar]
  9. Davis M. A., Martin K. A., Austin S. J.. 1992; Biochemical activities of the ParA partition protein of the P1 plasmid. Mol Microbiol6:1141–1147[CrossRef]
    [Google Scholar]
  10. Del Solar G., Giraldo R., Ruiz-Echevarria M. J., Espinosa M., Diaz-Orejas R.. 1998; Replication and control of circular bacterial plasmids. Microbiol Mol Biol Rev62:434–464
    [Google Scholar]
  11. Fischer G., Holl A. C., Volff J. N., Vandewiele D., Decaris B., Leblond P.. 1998; Replication of the linear chromosomal DNA from the centrally located oriC of Streptomyces ambofaciens revealed by PFGE gene dosage analysis. Res Microbiol149:203–210[CrossRef]
    [Google Scholar]
  12. Gavigan J. A., Ainsa J. A., Perez E., Otal I., Martin C.. 1997; Isolation by genetic labeling of a new mycobacterial plasmid, pJAZ38, from Mycobacterium fortuitum. J Bacteriol179:4115–4122
    [Google Scholar]
  13. Haeseleer F.. 1994; Structural instability of recombinant plasmids in mycobacteria. Res Microbiol145:683–687[CrossRef]
    [Google Scholar]
  14. Hanahan S.. 1983; Studies on transformation of Escherichia coli with plasmids. J Mol Biol166:557–580[CrossRef]
    [Google Scholar]
  15. Hinnebusch J., Tilly K.. 1993; Linear plasmids and chromosomes in bacteria. Mol Microbiol10:917–922[CrossRef]
    [Google Scholar]
  16. Kalkus J., Dorrie C., Fischer D., Reh M., Schegel H. G.. 1993; The giant linear plasmid pHG207 from Rhodococcus sp. encoding hydrogen autotrophy: characterization of the plasmid and its termini. J Gen Microbiol139:2055–2065[CrossRef]
    [Google Scholar]
  17. Kesseler M., Dabbs E. R., Averhoff B., Gottschalk G.. 1996; Studies on the isopropylbenzene 2,3-dioxygenase and the 3-isopropylcatechol 2,3-dioxygenase genes encoded by the linear plasmid of Rhodococcus erythropolis BD2. Microbiology142:3241–3251[CrossRef]
    [Google Scholar]
  18. Kosono S., Maeda M., Fuji F., Arai H., Kudo T.. 1997; Three of the seven bphC genes of Rhodococcus erythropolis TA421, isolated from a termite ecosystem, are located on an indigenous plasmid associated with biphenyl degradation. Appl Environ Microbiol63:3282–3285
    [Google Scholar]
  19. Musialowski M. S., Flett F., Scott G. B., Hobbs G., Smith C., Oliver S. G.. 1994; Functional evidence that the prinicipal DNA replication origin of the Streptomyces coelicolor chromosome is close to the dnaAgyrB region. J Bacteriol176:5123–5125
    [Google Scholar]
  20. Pelicic V., Reyrat J. M., Gicquel B.. 1996; Generation of unmarked directed mutations in mycobacteria, using sucrose counter-selectable suicide vectors. Mol Microbiol20:919–925[CrossRef]
    [Google Scholar]
  21. Picardeau M., Vincent V.. 1997; Characterization of large linear plasmids in mycobacteria. J Bacteriol179:2753–2756
    [Google Scholar]
  22. Picardeau M., Vincent V.. 1998; Mycobacterial linear plasmids have an invertron-like structure related to other linear replicons in actinomycetes. Microbiology144:1981–1988[CrossRef]
    [Google Scholar]
  23. Polo S., Guerini O., Sosio M., Deho G.. 1998; Identification of two linear plasmids in the actinomycete Planobispora rosea. Microbiology144:2819–2825[CrossRef]
    [Google Scholar]
  24. Qin M., Taniguchi H., Mizuguchi Y.. 1994; Analysis of the replication region of a mycobacterial plasmid, pMSC262. J Bacteriol176:419–425
    [Google Scholar]
  25. Qin Z., Cohen S. N.. 1998; Replication at the telomeres of the Streptomyces linear plasmid pSLA2. Mol Microbiol28:893–903[CrossRef]
    [Google Scholar]
  26. Ranes M. G., Rauzier J., Lagranderie M., Gheorghiu M., Gicquel B.. 1990; Functional analysis of pAL5000, a plasmid from Mycobacterium fortuitum: construction of a ‘mini’ MycobacteriumEscherichia coli shuttle vector. J Bacteriol172:2793–2797
    [Google Scholar]
  27. Rauzier J., Moniz-Pereira J., Gicquel-Sanzey B.. 1988; Complete nucleotide sequence of pAL5000, a plasmid from Mycobacterium fortuitum. Gene71:315–321[CrossRef]
    [Google Scholar]
  28. Sakaguchi K.. 1990; Invertrons, a class of structurally and functionally related genetic elements that includes linear DNA plasmids, transposable elements, and genomes of adenotype viruses. Microbiol Rev54:66–74
    [Google Scholar]
  29. Salas M.. 1991; Protein-priming of DNA replication. Annu Rev Biochem60:39–71[CrossRef]
    [Google Scholar]
  30. Sanger F., Nicklen S., Coulson A. R.. 1977; DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA74:5463–5467[CrossRef]
    [Google Scholar]
  31. Shiffman D., Cohen S. N.. 1992; Reconstruction of a Streptomyces linear replicon from separately cloned DNA fragments: existence of a cryptic origin of circular replication within the linear plasmid. Proc Natl Acad Sci USA89:6129–6133[CrossRef]
    [Google Scholar]
  32. Snapper S. B., Melton R. E., Mustafa S., Kieser T., Jacobs W. R. Jr. 1990; Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Mol Microbiol4:1911–1919[CrossRef]
    [Google Scholar]
  33. Stolt P., Stoker N. G.. 1996; Functional definition of regions necessary for replication and incompatibility in the Mycobacterium fortuitum plasmid pAL5000. Microbiology142:2795–2802[CrossRef]
    [Google Scholar]
  34. Zakrzewska-Czerwinska J., Schrempf H.. 1992; Characterization of an autonomously replicating region from the Streptomyces lividans chromosome. J Bacteriol147:2688–2693
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/micro/10.1099/00221287-146-2-305
Loading
/content/journal/micro/10.1099/00221287-146-2-305
Loading

Data & Media loading...

Most cited this month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error