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Volume 146, Issue 2

Regulation of the transport system for C-dicarboxylic acids in Free

Abstract

Transport systems for C-dicarboxylates, such as malate, fumarate and succinate, are poorly understood in Gram-positive bacteria. The whole genome sequence of revealed two genes, and , whose deduced products are highly homologous to binding proteins and transporters for C-dicarboxylates in Gram-negative bacteria. Between and , genes and encoding a sensor–regulator pair, were located. Inactivation of each one of the genes caused a deficiency in utilization of fumarate or succinate but not of malate. Expression of , encoding a putative transporter, was stimulated in a minimal salt medium containing 005% yeast extract but repressed by the addition of malate to the medium. Inactivation of the putative sensor–regulator pair or solute-binding protein, or , caused complete loss of expression. The utilization of fumarate and stimulation of expression resumed in a null mutant in which were overproduced. Based on these observations, together with analysis of the sequence similarities of the deduced product, we conclude that YdbH is a C-dicarboxylate-transport protein and its expression is regulated by a C-dicarboxylate sensor kinase–regulator pair, YdbF and YdbG. Furthermore, it is suggested that YdbE does not directly participate in transport of C-dicarboxylates, but plays a sensory role in the – two-component system, giving rise to specificity or increased efficiency to the system. Deletion analysis of the promoter region of revealed that a direct repeat sequence was required for the activation of expression. A catabolite-responsive element (CRE) was also found in the −10 region of the promoter, suggesting negative regulation by a CRE-binding protein.

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  • Accepted:
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Keyword(s): Bacillus subtilis , C4-dicarboxylate , Dct, dicarboxylic acid transport , solute-binding protein , transporter and two-component regulatory system
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2000-02-01
2024-04-25
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Regulation of the transport system for C4-dicarboxylic acids in Bacillus subtilis
Microbiology 146, 263 (2000); https://doi.org/10.1099/00221287-146-2-263
/content/journal/micro/10.1099/00221287-146-2-263
/content/journal/micro/10.1099/00221287-146-2-263
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