1887

Abstract

Mutations conferring resistance to the antibiotic rifampicin (Rif) occur at specific sites within the β subunit of the prokaryotic RNA polymerase. Rif mutants of are frequently altered in the elongation and termination of transcription. Rif mutations were isolated in and their effects on transcription elongation factor NusG and Rho-dependent termination were investigated. RNase protection assay, Northern analysis and the expression of fusions in cells with an inducible NusG suggested the gene was autoregulated at the level of transcription. Rif mutations that changed residue Q469 to a basic residue (Q469K and Q469R) enhanced autoregulation of . A mutant expressing a truncated form of NusG, due to a nonsense mutation within the gene, was isolated on the basis of the loss of autoregulation. The mechanism of autoregulation was found to be independent both of transcription termination factor Rho and of the promoter transcribing Autoregulation required sequences within the 5′ coding sequence of the gene or immediately upstream. This is the first evidence from any bacterium that Rif RNA polymerases can display altered transcription regulation by NusG.

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2000-12-01
2020-03-28
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