1887

Abstract

A3(2) strain M145 has eight chitinase genes scattered on the chromosome: six genes for family 18 (, , , , and ) and two for family 19 ( and ). In this study, the expression and regulation of these genes were investigated. The transcription of five of the genes (, , , and ) was induced in the presence of colloidal chitin while that of the other three genes (, and ) was not. The transcripts of the five induced genes increased and reached their maximum at 4 h after the addition of colloidal chitin, all showing the same temporal patterns. The induced levels of the transcripts of were significantly lower than those of the other four genes. Dynamic analysis of the transcripts of the genes indicated that and were induced more strongly than and . Addition of chitobiose also induced transcription of the genes, but significantly earlier than did colloidal chitin. When cells were cultured in the presence of colloidal chitin, an exponential increase of chitobiose concentration in the culture supernatant was observed prior to the induced transcription of the genes. This result, together with the immediate effect of chitobiose on the induction, suggests that chitobiose produced from colloidal chitin is involved in the induction of transcription of the genes. The transcription of the five genes was repressed by glucose. This repression was apparently mediated by the glucose kinase gene .

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2000-11-01
2019-10-16
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