1887

Abstract

The GenBank accession number for the sequence reported in this paper is U87924.

Colony pleomorphism, or phase variation, expressed by entomopathogenic bacteria belonging to the genus , is an important factor which determines the association of the bacteria with their nematode symbiont and the outcome of infection of susceptible insect larvae by the bacterium– nematode parasitic complex. The mechanism underlying phase variation is unknown. To determine whether RecA-mediated processes are linked to phase variation, the gene of was cloned and sequenced. When expressed in a -deleted strain of , the clone was able to complement the loss of RecA function. . chromosomal insertion mutants showed increased sensitivity to UV. Phase 1 forms did not show altered ability to convert to phase 2 and no significant differences in expression of other phase-dependent characteristics, including phospholipase C, haemolysin, protease, antibiotic activity and Congo Red binding, were noted. Furthermore, the LD of the insertion mutant for larvae was not significantly different from that of wild-type strains. From these data the authors conclude that is unlikely to be involved in phase variation, the expression of phase-dependent characteristics, or virulence factors involved in killing of susceptible larvae.

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2000-11-01
2020-01-29
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