1887

Abstract

The GenBank accession number for the sequence reported in this paper is Y17900.

The genes from the cluster of NCFB 2393 involved in the biosynthesis of EPS were identified, cloned and nucleotide sequenced. The complete cluster is contained on an ∼112 kb chromosomal region which contains 12 ORFs, including the previously cloned genes. Functions were assigned to some of the predicted gene products on the basis of homology to known sequences as follows: encodes a protein thought to be involved in the polymerization and export of the polysaccharide; , , , , and encode putative sugar transferases. Two insertion sequences, IS and IS, were identified within and flanking the 3′ end of the cluster respectively. Analysis of the expression of the gene in demonstrated that it encodes a glucose-1-phosphate transferase; the enzyme which catalyses the first step in EPS biosynthesis in NCFB 2393.

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2000-11-01
2021-10-28
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