1887

Abstract

To investigate the role of mitogenic factor (MF) in streptococcal pathogenesis, the structural gene () encoding this protein was disrupted in a clinical isolate of H293, to yield the isogenic mutant H363. Growth in enriched broth and on blood agar was unaffected by disruption of . Cell-free broth supernatants from H293 and H363 demonstrated identical promitogenic activities when co-incubated with human peripheral blood mononuclear cells, even when diluted 100000-fold, showing that MF is not a major streptococcal mitogen compared with other secreted superantigens. Disruption of resulted in complete loss of DNase B production and detectable DNase activity in H363 compared with the parent strain, confirming that the single gene , which is present in all group A streptococcal M serotypes studied, encodes DNase B. Despite loss of DNase activity, the virulence of in a mouse model of necrotizing fasciitis and myositis was unaffected.

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2000-11-01
2019-12-05
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