1887

Abstract

To determine if cellular functions of the phosphatidylinositol 3-kinase CaVps34p are related to processes governing pathogenicity, both copies of the gene were sequentially disrupted. Homozygous deletion of resulted in a mutant strain which exhibited defects not only in intracellular vesicle transport processes but also in morphogenesis. The null mutant was unable to form hyphae on different solid media whilst showing a significantly delayed yeast-to-hyphae transition in liquid media. In addition, the mutant was rendered hypersensitive to temperature and osmotic stresses and had a strongly decreased ability to adhere to mouse fibroblast cells compared to the wild-type strain SC5314. Finally, evidence was obtained that is essential for pathogenicity of as the null mutant was shown to be avirulent in a mouse model of systemic infection. pathogenicity was restored to a near wild-type degree upon reintroduction of into the chromosome of the null mutant, demonstrating that the observed avirulence corresponded to the loss of . Thus, the results suggest that may serve as a potential target for antifungal drugs.

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2000-11-01
2020-01-24
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