1887

Abstract

In the past it has been reproducibly demonstrated that 37 °C-grown DBY747 yeast cells have 29% more unsaturated fatty acids and a 3 °C higher maximal heat shock response (HSR) than their 25 °C counterparts. Suddenly the HSR and lipid profiles of cells grown at 25 °C and 37 °C became indistinguishable from one another. This paper reports an aberrantly high level of unsaturated fatty acids and an abnormally insensitive HSR in cells grown at 25 °C in yeast nitrogen base (YNB) that has been reconstituted from dehydrated medium packaged in ’new’ plastic containers. Effective even at a 1:600 dilution of reconstituted medium in laboratory-made YNB, the ’active ingredient’ was identified using a combination of HPLC and mass spectroscopy as dioctyl phthalate (a plasticising agent). Furthermore, the same levels of increase in the percentage of unsaturated fatty acids and decrease in the sensitivity of HSR were found in cells grown in laboratory-made YNB that contained as little as 36 μM pure dioctyl phthalate. This compound nevertheless failed to elicit an observable effect on cellular growth rate at levels up to and including 144 μM. These results suggest that dioctyl phthalate causes yeast cells to accumulate high levels of unsaturated fatty acids with a concomitant decrease in the sensitivity of the HSR, without compromising overall cellular function. They also support earlier work that suggested that the HSR is exquisitely sensitive to the level of unsaturated fatty acids present in yeast cells.

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2000-10-01
2019-10-15
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References

  1. Bojes, H. K. & Thurman, R. G. ( 1996; ). Potent peroxisome proliferators inhibit beta-oxidation in the isolated perfused rat liver. Toxicol Appl Pharmacol 140, 322-327.[CrossRef]
    [Google Scholar]
  2. Carratu, L., Franceschelli, S., Pardini, C. L., Kobayashi, G. S., Horvath, I., Vigh, L. & Maresca, B. ( 1996; ). Membrane lipid perturbation modifies the set point of the temperature of heat shock response in yeast. Proc Natl Acad Sci U S A 93, 3870-3875.[CrossRef]
    [Google Scholar]
  3. Chatterjee, M. T., Khalawan, S. A. & Curran, B. P. G. ( 1997; ). Alterations in cellular lipids may be responsible for the transient nature of the yeast heat shock response. Microbiology 143, 3063-3068.[CrossRef]
    [Google Scholar]
  4. Coote, P. J., Cole, M. C. & Jones, M. V. ( 1991; ). Induction of increased thermotolerance in Saccharomyces cerevisiae may be triggered by a mechanism involving intracellular pH. J Gen Microbiol 137, 1701-1708.[CrossRef]
    [Google Scholar]
  5. Coote, P. J., Jones, M. V., Seymour, I. J., Rowe, D. L., Ferdinando, D. P., McArthur, A. J. & Cole, M. B. ( 1994; ). Activity of plasma membrane H+-ATPase is a key physiological determinant of thermotolerance in Saccharomyces cerevisiae. Microbiology 140, 1881-1890.[CrossRef]
    [Google Scholar]
  6. Cronan, J. E. ( 1978; ). Molecular biology of bacterial membrane lipids. Annu Rev Biochem 47, 163-189.[CrossRef]
    [Google Scholar]
  7. Filipits, M., Simon, M. M., Rapatz, W., Hamilton, B. & Ruis, H. ( 1993; ). A Saccharomyces cerevisiae upstream activating sequence mediates induction of peroxisome proliferation by fatty acids. Gene 132, 49-55.[CrossRef]
    [Google Scholar]
  8. Hossack, J. A. & Rose, A. H. ( 1976; ). Fragility of plasma membranes in Saccharomyces cerevisiae enriched with different sterols. J Bacteriol 127, 67-75.
    [Google Scholar]
  9. Hunter, T. & Plowman, G. D. ( 1997; ). The protein kinases of budding yeast: six score and more. Trends Biochem Sci 22, 18-21.
    [Google Scholar]
  10. Hunter, K. & Rose, A. H. ( 1972; ). Lipid composition of Saccharomyces cerevisiae as influenced by growth temperature. Biochim Biophys Acta 260, 639-653.[CrossRef]
    [Google Scholar]
  11. Kamada, Y., Jung, U. S., Piotrowski, J. & Levin, D. E. ( 1995; ). The protein kinase C-activated MAP kinase pathway of Saccharomyces cerevisiae mediates a novel aspect of the heat shock response. Genes Dev 9, 1559-1571.[CrossRef]
    [Google Scholar]
  12. Kondo, K. & Inouye, M. ( 1991; ). TIP1, a cold shock-inducible gene of Saccharomyces cerevisiae. J Biol Chem 266, 17537-17544.
    [Google Scholar]
  13. Latruffe, N. & Vamecq, J. ( 1997; ). Peroxisome proliferators and peroxisome proliferator activated receptors (PPARs) as regulators of lipid metabolism. Biochimie 79, 81-94.[CrossRef]
    [Google Scholar]
  14. Livneh, E. & Fishman, D. D. ( 1997; ). Linking protein kinase C to cell-cycle control. Eur J Biochem 248, 1-9.[CrossRef]
    [Google Scholar]
  15. Mager, W. H. & De Kruijff, A. J. J. ( 1995; ). Stress-induced transcriptional activation. Microbiol Rev 59, 506-531.
    [Google Scholar]
  16. Miller, M. J., Xuong, N. & Geiduschek, E. P. ( 1979; ). A response of protein synthesis to temperature shift in the yeast Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 76, 5222-5225.[CrossRef]
    [Google Scholar]
  17. Okuyama, H., Saito, M., Joshi, V. C., Gunsberg, S. & Wakil, S. L. ( 1979; ). Regulation by temperature of the chain length of fatty acids in yeast. J Biol Chem 254, 12281-12284.
    [Google Scholar]
  18. Panaretou, B. & Piper, P. W. ( 1990; ). Plasma-membrane ATPase action affects several stress tolerances of Saccharomyces cerevisiae and Schizosaccharomyces pombe as well as the extent and duration of the heat shock response. J Gen Microbiol 136, 1763-1770.[CrossRef]
    [Google Scholar]
  19. Panaretou, B. & Piper, P. W. ( 1992; ). The plasma membrane of yeast acquires a novel heat shock protein (Hsp30) and displays a decline in proton-pumping ATPase levels in response to both heat shock and the entry to stationary phase. Eur J Biochem 206, 635-640.[CrossRef]
    [Google Scholar]
  20. Parsell, D. A. & Lindquist, S. ( 1993; ). The function of heat shock proteins in stress tolerance: degradation and reactivation of damaged proteins. Annu Rev Genet 27, 437-496.[CrossRef]
    [Google Scholar]
  21. Rottensteiner, H., Kal, A. J., Filipits, M., Binder, M., Hamilton, B., Tabak, H. F. & Ruis, H. ( 1996; ). Pip2p: a transcriptional regulator of peroxisome proliferation in the yeast Saccharomyces cerevisiae. EMBO J 15, 2924-2934.
    [Google Scholar]
  22. Slater, M. R. & Craig, E. A. ( 1987; ). Transcriptional regulation of an Hsp70 heat shock gene in the yeast Saccharomyces cerevisiae. Mol Cell Biol 7, 1906-1916.
    [Google Scholar]
  23. Small, G. M., Karpichev, I. V. & Luo, Y. ( 1997; ). Regulation of peroxisomal fatty acyl-CoA oxidase in the yeast, Saccharomyces cerevisiae. Adv Exp Med Biol 422, 157-166.
    [Google Scholar]
  24. Sorger, P. K. ( 1990; ). Yeast heat shock factor contains separable transient and sustained response transcriptional activators. Cell 62, 793-805.[CrossRef]
    [Google Scholar]
  25. Sorger, P. K. ( 1991; ). Heat shock factor and the heat shock response. Cell 65, 363-366.[CrossRef]
    [Google Scholar]
  26. Sorger, P. K. & Pelham, H. R. B. ( 1987; ). Purification and characterization of a heat shock element binding protein from yeast. EMBO J 6, 3035-3041.
    [Google Scholar]
  27. Sorger, P. K., Lewis, M. J. & Pelham, H. R. B. ( 1987; ). Heat shock factor is regulated differently in yeast and HeLa cells. Nature 329, 81-84.[CrossRef]
    [Google Scholar]
  28. Suutari, M., Liukkonen, K. & Laakso, S. ( 1990; ). Temperature adaptation in yeasts: the role of fatty acids. J Gen Microbiol 136, 1469-1474.[CrossRef]
    [Google Scholar]
  29. Veenhuis, M., Mateblowski, M., Kunau, W. H. & Harder, W. ( 1987; ). Proliferation of microbodies in Saccharomyces cerevisiae. Yeast 3, 77-84.[CrossRef]
    [Google Scholar]
  30. Vigh, L., Maresca, B. & Harwood, J. L. ( 1998; ). Does the membrane’s physical state control the expression of heat shock and other genes? Trends Biochem Sci 10, 369-374.
    [Google Scholar]
  31. Weitzel, G., Pilatus, U. & Rensing, L. ( 1987; ). The cytoplasmic pH, ATP content and total protein synthesis rate during heat shock inducing treatments in yeast. Exp Cell Res 170, 64-79.[CrossRef]
    [Google Scholar]
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