1887

Abstract

Normally glucosamine (GlcN) is not a substrate for EIICB of the glucose phosphotransferase system (PTS), encoded by , but it is transported by the mannose (Man) PTS, encoded by . A mutation, , has been described in which allows a strain mutated in the Man PTS to grow on GlcN. The mutation was mapped to the region and was proposed to make expression constitutive. Transcription of is regulated by the repressor Mlc so that mutations in enhance the expression of . An mutation, however, is not sufficient to allow good growth on GlcN, unlike the mutation. The mutation is shown to enhance expression of even in the absence of any PTS sugar transport, but the increase is greater in the presence of GlcN or Man. The mutation also increases expression of the and operons, which are both regulated by Mlc. The mutation was sequenced and two mutations were found: one, G176D, within the IIC membrane domain and the second, E472K, within the soluble IIB domain of PtsG. The cloned UmgC allele shows the enhanced transport and regulatory characteristics of the chromosomal mutation. Analysis of the two mutations present individually on plasmids shows that the IIC mutation is responsible for both the effect on sugar specificity and regulation.

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2000-10-01
2019-10-16
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