1887

Abstract

The promoter of the nitric oxide reductase operon () has been characterized by primer extension and deletion analysis. A major transcript that is detectable only in anaerobically grown cells initiates 435 bp downstream of the centre of a putative binding site for the transcription factor NNR (nitrite and nitric oxide reductase regulator, which is known to regulate expression). A minor transcript initiates 121 bp upstream of the major transcript and is detectable in cells grown aerobically or anaerobically. Deletion derivatives of the promoter region were constructed and analysed using transcriptional fusions to the reporter gene . Expression patterns from promoter deletions in a wild-type strain and an mutant confirmed that the minor transcript is NNR independent, and makes a small contribution to expression under both aerobic and anaerobic growth conditions. A deletion derivative truncated to within 7 bp of the putative NNR-binding site showed a near wild-type response to anaerobic growth, showing that no upstream DNA sequences are required for activation of the major promoter. Site-directed mutagenesis of the putative NNR-binding site confirmed that this is the major acting sequence mediating the anaerobic inducibility of expression.

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2000-10-01
2020-04-07
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