1887

Abstract

is the cause of a serious tick-borne disease of domestic ruminants, known as heartwater or cowdriosis. The organism belongs to the tribe , which contains obligate intracellular pathogens, causing several important animal and human diseases. Although a few genes have been cloned and sequenced, very little is known about the size, gross structure and organization of the genome. This paper presents a complete physical map and a preliminary genetic map for . Chromosomal DNA was examined by PFGE and Southern hybridization. PFGE analysis revealed that has a circular chromosome approximately 1576 kb in size. A physical map was derived by combining the results of PFGE analysis of DNA fragments resulting from digestion of the whole genome with I, II and I and Southern hybridization analysis with a series of gene probes and isolated macrorestriction fragments. A genetic map for with a mean resolution of 290 kb was established, the first for a member of the . A total of nine genes or cloned DNA fragments were mapped to specific I, II and I fragments, including the major antigenic protein gene,

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2000-10-01
2020-03-29
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