1887

Abstract

FtsY (Srb) is a homologue of the α subunit of the receptor for mammalian signal-recognition particle (SRP) and is essential for protein secretion and vegetative cell growth. The gene is expressed during both the exponential phase and sporulation. In vegetative cells, is transcribed with two upstream genes, and , that are under the control of the major transcription factor σ. During sporulation, Northern hybridization detected mRNA in wild-type cells, but not in sporulating cells of σ and mutants. Therefore, is solely expressed during sporulation from a σ- and GerE-controlled promoter that is located immediately upstream of inside the gene. To examine the role of FtsY during sporulation, the strain ISR39 was constructed, a conditional mutant in which expression can be shut off during spore formation but not during the vegetative state. Electron microscopy showed that the outer coat of ISR39 spores was not completely assembled and immunoelectron microscopy localized FtsY to the inner and outer coats of wild-type spores.

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2000-10-01
2019-10-20
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